Department of oncology, Tianjin Baodi hospital, Baodi Clinical College of Tianjin Medical University, China.
Department of thoracic surgery, Tianjin Baodi hospital, Baodi Clinical College of Tianjin Medical University, China.
Clin Chim Acta. 2020 Mar;502:55-65. doi: 10.1016/j.cca.2019.11.022. Epub 2019 Dec 9.
Urinary proteins could be useful as markers for the detection of non-small-cell lung cancer (NSCLC). We investigated the levels of two different proteins in urine samples from NSCLC patients and assessed their diagnostic value.
Urinary plasminogen (PLG) and fibrinogen gamma chain (FGG) levels in 112 NSCLC patients and 197 controls were detected using enzyme linked immunosorbent assay (ELISA). The expression of FGG and PLG in 20 NSCLC tissues and paired adjacent non-tumour tissues were detected through immunohistochemistry. The diagnostic value of FGG and PLG for NSCLC was evaluated through a receiver operating characteristic curve (ROC).
PLG and FGG were significantly elevated in NSCLC tissues vs paired adjacent non-tumour tissues (p = 0.000) and in urinary samples from NSCLC patients vs healthy controls (p = 0.000). The expression level of PLG in urinary samples was related only to the histological type (p = 0.001). Further, ROC curve analysis revealed that PLG, FGG, and their combination could distinguish NSCLC and its subtypes from healthy controls with an AUC ranging from 0.827 to 0. 947. By comparing urine samples with matching plasma CEA from NSCLC stage I-IV patients (n = 81) and healthy controls (n = 31), the combination of CEA with PLG or FGG showed that the AUC was 0.889 and 0.806, respectively, which is superior to a single biomarker alone.
These two urinary proteins could serve as potential markers for the diagnosis of NSCLC.
尿液蛋白可用作检测非小细胞肺癌（NSCLC）的标志物。我们研究了 NSCLC 患者尿液样本中两种不同蛋白的水平，并评估了它们的诊断价值。
采用酶联免疫吸附试验（ELISA）检测 112 例 NSCLC 患者和 197 例对照者尿液中尿纤溶酶原（PLG）和纤维蛋白原γ链（FGG）的水平。通过免疫组织化学检测 20 例 NSCLC 组织及其配对的癌旁非肿瘤组织中 FGG 和 PLG 的表达。通过受试者工作特征曲线（ROC）评估 FGG 和 PLG 对 NSCLC 的诊断价值。
PLG 和 FGG 在 NSCLC 组织与配对的癌旁非肿瘤组织（p=0.000）以及 NSCLC 患者与健康对照者的尿液样本中均显著升高（p=0.000）。尿液样本中 PLG 的表达水平仅与组织学类型有关（p=0.001）。此外，ROC 曲线分析显示，PLG、FGG 及其组合可区分 NSCLC 及其亚型与健康对照者，AUC 范围为 0.827 至 0.947。通过比较 NSCLC Ⅰ-Ⅳ期患者（n=81）和健康对照者（n=31）的尿液样本与配对的血浆 CEA，CEA 与 PLG 或 FGG 的组合显示 AUC 分别为 0.889 和 0.806，优于单一生物标志物。
这两种尿蛋白可作为 NSCLC 诊断的潜在标志物。
