Aree Tanatchaporn, Chaichit Siripat, Junlatat Jintana, Kiattisin Kanokwan, Intharuksa Aekkhaluck
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Faculty of Thai Traditional and Alternative Medicine, Ubon Ratchathani Rajabhat University, Ubon Ratchathani 34000, Thailand.
Int J Mol Sci. 2025 Aug 9;26(16):7716. doi: 10.3390/ijms26167716.
L. (Fabaceae: Faboideae), traditionally used as green manure due to its nitrogen-fixing capacity, also exhibits therapeutic potential for conditions such as anemia and psoriasis. However, its cosmetic applications remain largely unexplored. This study examined the phytochemical profiles and biological activities of ethanolic extracts from the root, flower, and leaf of , focusing on their potential use in cosmetic formulations. Soxhlet extraction with 95% ethanol was employed. Among the extracts, the leaf showed the highest total flavonoid content, while the root contained the highest total phenolic content. The root extract demonstrated the strongest antioxidant activity, as assessed by DPPH, FRAP, and lipid peroxidation assays, along with significant anti-tyrosinase and anti-aging effects via collagenase and elastase inhibition. LC-MS/QTOF analysis identified genistein and kaempferol as the major bioactive constituents in the root extract. Molecular docking confirmed their strong interactions with enzymes associated with skin aging. Additionally, the root extract exhibited notable anti-inflammatory activity. These results suggest that root extract is a promising multifunctional natural ingredient for cosmetic applications due to its antioxidant, anti-tyrosinase, anti-aging, and anti-inflammatory properties.
L.（豆科：蝶形花亚科），因其固氮能力传统上用作绿肥，对贫血和牛皮癣等病症也具有治疗潜力。然而，其在化妆品中的应用在很大程度上仍未得到探索。本研究考察了L.根、花和叶乙醇提取物的植物化学特征和生物活性，重点关注它们在化妆品配方中的潜在用途。采用95%乙醇进行索氏提取。在提取物中，叶的总黄酮含量最高，而根的总酚含量最高。通过DPPH、FRAP和脂质过氧化测定评估，根提取物表现出最强的抗氧化活性，同时通过抑制胶原酶和弹性蛋白酶具有显著的抗酪氨酸酶和抗衰老作用。LC-MS/QTOF分析确定染料木黄酮和山奈酚为根提取物中的主要生物活性成分。分子对接证实了它们与皮肤衰老相关酶的强相互作用。此外，根提取物表现出显著的抗炎活性。这些结果表明，L.根提取物因其抗氧化、抗酪氨酸酶、抗衰老和抗炎特性，是一种有前景的多功能天然化妆品成分。
