Phumat Pimpak, Chaichit Siripat, Potprommanee Siriporn, Preedalikit Weeraya, Sainakham Mathukorn, Poomanee Worrapan, Chaiyana Wantida, Kiattisin Kanokwan
Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Cosmetic Sciences, School of Pharmaceutical Sciences, University of Phayao, Phayao 56000, Thailand.
Foods. 2023 Sep 25;12(19):3555. doi: 10.3390/foods12193555.
peel, a type of postconsumer waste, is considered a source of beneficial phytochemicals. Therefore, it was subjected to investigation for biological activities in this study. peel was extracted using 95% /, 50% / ethanol and water. The obtained extracts were B95, B50 and BW. B95 had a high flavonoid content (212.88 ± 4.73 mg QE/g extract) and phenolic content (131.52 ± 0.38 mg GAE/g extract) and possessed high antioxidant activities as confirmed by DPPH, ABTS and lipid peroxidation inhibition assays. Moreover, B95 showed inhibitory effects against collagenase and hyaluronidase with values of 41.68 ± 0.92% and 29.17 ± 0.66%, which related to anti-aging activities. Via the HPLC analysis, one of the potential compounds found in B95 was rutin. Molecular docking has provided an understanding of the molecular mechanisms underlying the interaction of extracts with collagenase and hyaluronidase. All extracts were not toxic to fibroblast cells and did not irritate the hen's egg chorioallantoic membrane, which indicated its safe use. In conclusion, . peel extracts are promising potential candidates for further use as antioxidant and anti-aging agents in the food and cosmetic industries.
果皮是一种消费后废弃物，被认为是有益植物化学物质的来源。因此，本研究对其生物活性进行了调查。果皮用95%乙醇、50%乙醇和水进行提取。得到的提取物分别为B95、B50和BW。B95具有较高的黄酮含量（212.88±4.73毫克槲皮素当量/克提取物）和酚类含量（131.52±0.38毫克没食子酸当量/克提取物），并且通过DPPH、ABTS和脂质过氧化抑制试验证实具有较高的抗氧化活性。此外，B95对胶原酶和透明质酸酶表现出抑制作用，抑制率分别为41.68±0.92%和29.17±0.66%，这与抗衰老活性相关。通过高效液相色谱分析，发现B95中一种潜在的化合物是芦丁。分子对接提供了对提取物与胶原酶和透明质酸酶相互作用的分子机制的理解。所有提取物对成纤维细胞无毒，对鸡胚尿囊膜无刺激，表明其使用安全。总之，果皮提取物有望成为食品和化妆品行业中进一步用作抗氧化剂和抗衰老剂的潜在候选物。
