Cieśla Marek, Darmochwał-Kolarz Dorota, Pałka Anna, Łagowska Katarzyna, Tabarkiewicz Jacek, Kolarz Bogdan
Institute of Medical Sciences, Medical College of Rzeszow University, Rzeszów, Poland.
Institute of Medical Sciences, Medical College of Rzeszow University, Rzeszów, Poland
Pol Arch Intern Med. 2024 Dec 19;134(12). doi: 10.20452/pamw.16866. Epub 2024 Oct 30.
Long noncoding RNAs (lncRNAs) have long been considered molecular noise within the transcriptome, but over time it has been shown that they perform many important biological functions and are associated with various inflammatory and autoimmune diseases, including rheumatoid arthritis (RA).
The aim of this study was to evaluate the association between the circulating lncRNAs and RA activity.
The study included 63 patients with well‑established RA (median disease duration of 12 years), at a mean (SD) age of 51.7 (13) years, 88.9% women, and 25 healthy controls (HCs) at a mean (SD) age 51.8 (8.2) years, 80% women. Quantitative real‑time polymerase chain reaction was used to evaluate the plasma concentration levels of 6 lncRNAs: PACERR, NEAT1, HOTTIP, GAS5, MALAT1, and HIX003209.
Among the tested molecules, 5 targets (PACERR, NEAT1, HOTTIP, GAS5, and HIX003209) had lower concentrations in the RA patients than in the HCs. LncRNAs for NEAT1, PACERR, and GAS5 showed differences between the severe disease activity group (the 28 joint disease activity score [DAS28] / erythrocyte sedimentation rate >5.1) and HCs (P = 0.02, P = 0.003, and P = 0.04, respectively). The multiple linear regression analysis indicated that GAS5 had the greatest impact on the disease activity based on DAS28 (P = 0.01).
Circulating plasma lncRNAs may be considered as supporting molecular markers associated with RA activity and may be useful in identifying the disease exacerbation.
长期以来，长链非编码RNA（lncRNAs）一直被视为转录组中的分子噪音，但随着时间的推移，已证明它们具有许多重要的生物学功能，并与包括类风湿性关节炎（RA）在内的各种炎症和自身免疫性疾病相关。
本研究旨在评估循环lncRNAs与RA活动之间的关联。
该研究纳入了63例确诊为RA的患者（疾病中位持续时间为12年），平均（标准差）年龄为51.7（13）岁，女性占88.9%，以及25名健康对照者（HCs），平均（标准差）年龄为51.8（8.2）岁，女性占80%。采用定量实时聚合酶链反应评估6种lncRNAs（PACERR、NEAT1、HOTTIP、GAS5、MALAT1和HIX003209）的血浆浓度水平。
在测试的分子中，5个靶点（PACERR、NEAT1、HOTTIP、GAS5和HIX003209）在RA患者中的浓度低于HCs。NEAT1、PACERR和GAS5的lncRNAs在疾病活动严重组（28关节疾病活动评分[DAS28]/红细胞沉降率>5.1）和HCs之间存在差异（分别为P = 0.02、P = 0.003和P = 0.04）。多元线性回归分析表明，基于DAS28，GAS5对疾病活动的影响最大（P = 0.01）。
循环血浆lncRNAs可被视为与RA活动相关的辅助分子标志物，可能有助于识别疾病加重情况。
