Fu Qinchao, Wen Xinlan, Tang Man, Zhao Xin, Liu Fang
Natural Sciences Museum of Leshan Normal University, Key Laboratory of Sichuan Province for Bamboo Pests Control and Resource Development, Leshan Normal University, Leshan 614000, China.
College of Life Science and Agri-Forestry, Southwest University of Science and Technology, Mianyang 621000, China.
Genes (Basel). 2025 Jul 23;16(8):855. doi: 10.3390/genes16080855.
Moso bamboo (), the most widely distributed bamboo species in China, is valued for both its shoots and timber. This species often faces challenges from high-temperature stress. To cope with this stress, Moso bamboo has evolved various adaptive mechanisms at the physiological and molecular levels. Although numerous studies have revealed that a large number of transcription factors (TFs) and genes play important roles in the regulatory network of plant heat stress responses, the regulatory network involved in heat responses remains incompletely understood. In this study, Moso bamboo was placed in a high-temperature environment of 42 °C for 1 h and 24 h, and transcriptome sequencing was carried out to accurately identify key molecules affected by high temperature and their related biological pathways. Through a differential expression analysis, we successfully identified a series of key candidate genes and transcription factors involved in heat stress responses, including members of the ethylene response factor, HSF, WRKY, MYB, and bHLH families. Notably, in addition to traditional heat shock proteins/factors, multiple genes related to lipid metabolism, antioxidant enzymes, dehydration responses, and hormone signal transduction were found to play significant roles in heat stress responses. To further verify the changes in the expression of these genes, we used qRT-PCR technology for detection, and the results strongly supported their key roles in cellular physiological processes and heat stress responses. This study not only deepens our understanding of plant strategies for coping with and defending against extreme abiotic stresses but also provides valuable insights for future research on heat tolerance in Moso bamboo and other plants.
毛竹是中国分布最广的竹种，其笋和竹材都具有很高的价值。该物种经常面临高温胁迫的挑战。为了应对这种胁迫，毛竹在生理和分子水平上进化出了各种适应机制。尽管大量研究表明，许多转录因子（TFs）和基因在植物热应激反应的调控网络中发挥着重要作用，但热反应所涉及的调控网络仍未完全被理解。在本研究中，将毛竹置于42℃的高温环境中1小时和24小时，并进行转录组测序，以准确鉴定受高温影响的关键分子及其相关生物学途径。通过差异表达分析，我们成功鉴定了一系列参与热应激反应的关键候选基因和转录因子，包括乙烯反应因子、热激因子、WRKY、MYB和bHLH家族的成员。值得注意的是，除了传统的热休克蛋白/因子外，还发现多个与脂质代谢、抗氧化酶、脱水反应和激素信号转导相关的基因在热应激反应中发挥重要作用。为了进一步验证这些基因表达的变化，我们使用qRT-PCR技术进行检测，结果有力地支持了它们在细胞生理过程和热应激反应中的关键作用。本研究不仅加深了我们对植物应对和抵御极端非生物胁迫策略的理解，也为未来毛竹和其他植物耐热性的研究提供了有价值的见解。
