Chemical Process and Energy Resources Institute (CPERI), CERTH, 6th Km Harilaou-Thermi Road, 57001 Thermi, Thessaloniki, Greece.
Chemical Process and Energy Resources Institute (CPERI), CERTH, 6th Km Harilaou-Thermi Road, 57001 Thermi, Thessaloniki, Greece; Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str., Zografou Campus, Athens 15780, Greece.
Bioresour Technol. 2020 Oct;313:123599. doi: 10.1016/j.biortech.2020.123599. Epub 2020 Jun 4.
The valorization of lignocellulosic biomass towards the production of value-added products requires an efficient pretreatment/fractionation step. In this work we present a novel, acid-free, mildly oxidative organosolv delignification process -OxiOrganosolv- which employs oxygen gas to depolymerize and remove lignin. The results demonstrate that the OxiOrganosolv process achieved lignin removal as high as 97% in a single stage, with a variety of solvents; it was also efficient in delignifying both beechwood (hardwood) and pine (softwood), a task in which organosolv pretreatments have failed in the past. Minimal amounts of sugar degradation products were detected, while cellulose recovery was ~100% in the solid pulp. Enzymatic hydrolysis of pulps showed >80 wt% cellulose conversion to glucose. Overall, the OxiOrganosolv pretreatment has significant advantages, including high delignification efficiency of hardwood and softwood biomass, absence of acid homogeneous catalysis and all corresponding challenges involved, and close to zero losses of sugars to degradation products.
木质纤维素生物质的增值化需要高效的预处理/分离步骤。在这项工作中，我们提出了一种新颖的、无酸、轻度氧化的有机溶剂脱木质素方法-OxiOrganosolv-，该方法使用氧气气体来解聚和去除木质素。结果表明，OxiOrganosolv 工艺在单一阶段即可实现高达 97%的木质素去除率，适用于多种溶剂；它还能有效地脱除山毛榉木（硬木）和松木（软木）中的木质素，这是有机溶剂预处理过去未能完成的任务。检测到的糖降解产物很少，而固体纸浆中的纤维素回收率约为 100%。纸浆的酶水解显示出超过 80wt%的纤维素转化为葡萄糖。总的来说，OxiOrganosolv 预处理具有显著的优势，包括高效脱除硬木和软木生物质的木质素、不存在酸均相催化及其相关的所有挑战，以及几乎零糖降解产物损失。
