Henderson J A, Smey P, Cohen M S, Davis C P, Payer A F, Parkening T A, Warren M M
J Pediatr Surg. 1985 Dec;20(6):592-7. doi: 10.1016/s0022-3468(85)80005-1.
Recent studies of experimental testicular torsion in rats, rabbits, and guinea pigs have demonstrated conflicting evidence regarding contralateral testicular damage. Those studies in which cellular damage has been found are postulated to result from an immunological mechanism whereby the blood-testis barrier is disrupted with subsequent autoantibody formation. In this study, the histologic and immunologic effects of testicular torsion on the contralateral testicle were investigated in prepubertal Chinese hamsters. Four study groups were established; (1) Left orchiectomy only, (2) sham surgery (scrotal incision), (3) 720 degrees left testicular torsion with left orchiectomy 24 hours later, (4) 720 degrees torsion of left testicle with detorsion after 24 hours. The initial procedure was performed at 1 month of age with subsequent biopsies of the contralateral testicle at 1 week, 1 month, and 6 months after the initial procedure. Testicular tissue was examined for immunofluorescent activity using fluorescent labeled goat anti-hamster IgG. Positive controls were established by rabbit immunization (rabbit anti-hamster immunoglobulin) which was subsequently combined with fluorescent labeled goat antirabbit IgG. There was no appreciable difference in immunologic activity between control and experimental animals. Representative sections were examined histologically and no tubular damage was demonstrated and active spermatogenesis was noted at 6 months in all groups. We believe that our results support the premise that testicular torsion in the prepubertal period has no effect on the contralateral testicle.
最近对大鼠、兔子和豚鼠进行的实验性睾丸扭转研究，在对侧睾丸损伤方面得出了相互矛盾的证据。那些发现细胞损伤的研究推测是由一种免疫机制导致的，即血睾屏障被破坏，随后形成自身抗体。在本研究中，对青春期前中国仓鼠睾丸扭转对侧睾丸的组织学和免疫学影响进行了研究。设立了四个研究组：（1）仅左侧睾丸切除术；（2）假手术（阴囊切开）；（3）左侧睾丸720度扭转，24小时后行左侧睾丸切除术；（4）左侧睾丸扭转720度，24小时后解除扭转。初始手术在1月龄时进行，随后在初始手术后1周、1个月和6个月对侧睾丸进行活检。使用荧光标记的山羊抗仓鼠IgG检测睾丸组织的免疫荧光活性。通过兔免疫（兔抗仓鼠免疫球蛋白）建立阳性对照，随后将其与荧光标记的山羊抗兔IgG结合。对照动物和实验动物之间的免疫活性没有明显差异。对代表性切片进行组织学检查，未发现小管损伤，所有组在6个月时均观察到活跃的精子发生。我们认为我们的结果支持这样一个前提，即青春期前睾丸扭转对侧睾丸没有影响。
