Amnuaykan Piyatida, Anantaworasakul Pimporn, Lueadnakrob Kodpaka, Kunkul Pongsagon, Chokrungsarid Wilasinee, Thummanuwong Aiya, Juntrapirom Saranya, Kanjanakawinkul Watchara, Chaiyana Wantida
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Chulabhorn Royal Pharmaceutical Manufacturing Facilities by Chulabhorn Royal Academy, Chon Buri 20180, Thailand.
Pharmaceutics. 2025 Aug 20;17(8):1076. doi: 10.3390/pharmaceutics17081076.
This study aimed to develop a nanostructured lipid carrier (NLC) system incorporating a catechin-rich extract for topical cosmetic applications and to evaluate its physicochemical properties, release behavior, and skin retention performance. Blank NLCs were prepared using hot emulsification followed by sonication, with glyceryl monostearate, caprylic triglyceride, Poloxamer 188, and Tween 80 as the formulation components. NLCs with varying solid-to-liquid lipid ratios were developed while maintaining a constant total lipid content of 5% /. The formulations were characterized based on their particle size, polydispersity index (PDI), zeta potential, and physical stability, including stability after a heating-cooling cycle test. The effect of ultrasonication duration was also evaluated. The optimized NLC was then loaded with a extract and evaluated for in vitro release and skin retention using catechin as a marker. The NLC with a particle size of 235.5 ± 29.8 nm, a narrow PDI range of 0.382 ± 0.090, and a strong zeta potential of -29.8 ± 0.3 mV was selected for the incorporation of the extract. The extract-loaded NLC exhibited a sustained release over 24 h, significantly different from the extract solution ( < 0.05). Skin retention studies revealed that the NLC achieved approximately twice the catechin retention compared to the solution at the 1 h time point (1.30 ± 0.01% vs. 0.68 ± 0.03% /). The -extract-loaded NLC demonstrated favorable physicochemical properties, sustained release behavior, and enhanced skin retention. These findings support its potential as a promising topical delivery system for antioxidant-rich botanical extracts in cosmetic applications.
本研究旨在开发一种用于局部化妆品应用的、包含富含儿茶素提取物的纳米结构脂质载体（NLC）系统，并评估其物理化学性质、释放行为和皮肤保留性能。空白NLC采用热乳化后超声处理的方法制备，使用单硬脂酸甘油酯、辛酸甘油三酯、泊洛沙姆188和吐温80作为配方成分。在保持总脂质含量恒定为5%的情况下，开发了具有不同固液脂质比的NLC。根据其粒径、多分散指数（PDI）、zeta电位和物理稳定性（包括加热-冷却循环试验后的稳定性）对配方进行表征。还评估了超声处理持续时间的影响。然后用提取物加载优化后的NLC，并以儿茶素为标志物评估其体外释放和皮肤保留情况。选择粒径为235.5±29.8nm、PDI范围狭窄为0.382±0.090且zeta电位为-29.8±0.3mV的NLC用于掺入提取物。加载提取物的NLC在24小时内表现出持续释放，与提取物溶液有显著差异（P<0.05）。皮肤保留研究表明，在1小时时间点，NLC实现的儿茶素保留量约为溶液的两倍（1.30±0.01%对0.68±0.03%）。加载提取物的NLC表现出良好的物理化学性质、持续释放行为和增强的皮肤保留能力。这些发现支持了其作为化妆品应用中富含抗氧化剂植物提取物的有前景的局部递送系统的潜力。
