Hirai Nobumitsu, Miwa Yuhei, Hattori Shunta, Kanematsu Hideyuki, Ogawa Akiko, Iwata Futoshi
Department of Chemistry and Biochemistry, National Institute of Technology (KOSEN), Suzuka College, Shiroko-Cho, Suzuka 510-0294, Japan.
Department of Mechanical Engineering, Faculty of Engineering, Shizuoka University, 3-5-1 Johoku, Chuo-ku, Hamamatsu 432-8561, Japan.
Microorganisms. 2025 Jul 30;13(8):1779. doi: 10.3390/microorganisms13081779.
Biofilms cause a variety of problems, such as food spoilage, food poisoning, infection, tooth decay, periodontal disease, and metal corrosion, so knowledge on biofilm prevention and removal is important. A detailed observation of the three-dimensional structure of biofilms on the nanoscale is expected to provide insight into this. In this study, we report on the successful in situ nanoscale observations of a marine bacterial biofilm on glass in phosphate buffer solution (PBS) using both scanning ion conductance microscopy (SICM) and confocal laser scanning microscopy (CLSM) over the same area. By observing the same area by SICM and CLSM, we were able to clarify the three-dimensional morphology of the biofilm, the arrangement of bacteria within the biofilm, and the difference in local ion conductivity within the biofilm simultaneously, which could not be achieved by observation using a microscope alone.
生物膜会引发各种问题，如食物变质、食物中毒、感染、龋齿、牙周病和金属腐蚀，因此了解生物膜的预防和清除方法很重要。对生物膜在纳米尺度上的三维结构进行详细观察有望为此提供深入见解。在本研究中，我们报告了使用扫描离子电导显微镜（SICM）和共聚焦激光扫描显微镜（CLSM）在相同区域对磷酸盐缓冲溶液（PBS）中玻璃上的海洋细菌生物膜进行成功的原位纳米尺度观察。通过SICM和CLSM对同一区域进行观察，我们能够同时阐明生物膜的三维形态、生物膜内细菌的排列以及生物膜内局部离子电导率的差异，而仅使用显微镜观察是无法做到这一点的。
