El-Demerdash Azza SalahEldin, Sehim Amira E, Altamimi Abeer, Henidi Hanan, Mahran Yasmin, Dawwam Ghada E
Laboratory of Biotechnology, Department of Microbiology, Agricultural Research Centre (ARC), Animal Health Research Institute (AHRI), Zagazig 44516, Egypt.
Department of Botany and Microbiology, Faculty of Science, Benha University, Benha 13518, Egypt.
Microorganisms. 2025 Aug 4;13(8):1818. doi: 10.3390/microorganisms13081818.
The escalating global challenges of antimicrobial resistance (AMR) and cancer necessitate innovative therapeutic solutions from natural sources. This study investigated the multifaceted therapeutic potential of pigment-enriched plant extracts. We screened diverse plant extracts for antimicrobial and antibiofilm activity against multidrug-resistant bacteria and fungi. emerged as the most promising, demonstrating potent broad-spectrum antimicrobial and significant antibiofilm activity. Sub-inhibitory concentrations of robustly downregulated essential bacterial virulence genes and suppressed aflatoxin gene expression. Comprehensive chemical profiling via HPLC identified major anthocyanin glucosides, while GC-MS revealed diverse non-pigment bioactive compounds, including fatty acids and alcohols. Molecular docking suggested favorable interactions of key identified compounds (Cyanidin-3-O-glucoside and 1-Deoxy-d-arabitol) with outer membrane protein A (OmpA), indicating potential antiadhesive and antimicrobial mechanisms. Furthermore, exhibited selective cytotoxicity against MCF-7 breast cancer cells. These findings establish pigment-enriched extract as a highly promising, multi-functional source of novel therapeutics, highlighting its potential for simultaneously addressing drug resistance and cancer challenges through an integrated chemical, biological, and computational approach.
全球抗微生物药物耐药性（AMR）和癌症带来的挑战不断升级，需要从天然来源开发创新的治疗方案。本研究调查了富含色素的植物提取物的多方面治疗潜力。我们筛选了多种植物提取物，以检测其对耐多药细菌和真菌的抗菌及抗生物膜活性。[提取物名称]脱颖而出，表现出强大的广谱抗菌活性和显著的抗生物膜活性。亚抑制浓度的[提取物名称]能显著下调关键细菌毒力基因，并抑制黄曲霉毒素基因表达。通过高效液相色谱（HPLC）进行的综合化学分析确定了主要的花青素苷，而气相色谱 - 质谱联用（GC - MS）揭示了多种非色素生物活性化合物，包括脂肪酸和醇类。分子对接表明，关键鉴定化合物（矢车菊素 - 3 - O - 葡萄糖苷和1 - 脱氧 - D - 阿拉伯糖醇）与外膜蛋白A（OmpA）存在良好的相互作用，表明其潜在的抗黏附和抗菌机制。此外，[提取物名称]对MCF - 7乳腺癌细胞表现出选择性细胞毒性。这些发现确立了富含色素的[提取物名称]提取物作为一种极具前景的新型多功能治疗来源，突出了其通过综合化学、生物学和计算方法同时应对耐药性和癌症挑战的潜力。
