Anuarbekova Sandugash, Sadykov Azamat, Amangeldinova Dilnaz, Kanafina Marzhan, Sharova Darya, Alzhanova Gulzhan, Nurgaliyeva Rimma, Jumagaziyeva Ardak, Tynybayeva Indira, Zhumakaeva Aikumys, Rsaliyev Aralbek, Abduraimov Yergali, Kanafin Yerkanat N
Scientific and Analytical Center "Biomedpreparat" LLP, Stepnogorsk 021500, Kazakhstan.
Scientific Center for Anti-Infectious Drugs, Almaty 050000, Kazakhstan.
Microorganisms. 2025 Aug 6;13(8):1831. doi: 10.3390/microorganisms13081831.
Biotechnological research increasingly focuses on developing new drugs to counter the rise of antibiotic-resistant strains in hospitals. This study aimed to create bacterial lysates from antibiotic-resistant pathogens isolated from patients and medical instruments across hospital departments. Identification was performed based on morphological, cultural, and biochemical characteristics, as well as 16S rRNA gene sequencing using the BLAST algorithm. Strain viability was assessed using the Miles and Misra method, while sensitivity to eight antibacterial drug groups and biosafety between cultures were evaluated using agar diffusion. From 15 clinical sources, 25 pure isolates were obtained, and their phenotypic and genotypic properties were studied. Carbohydrate fermentation testing confirmed that the isolates belonged to the genera , , , , , , , and . The cultures exhibited good viability (10-10 CFU/mL) and compatibility with each other. Based on prevalence and clinical significance, three predominant hospital pathogens ( 12 BL, 3 BL, and 24 BL) were selected to develop a bacterial lysate consortium. Lysates were prepared with physical disruption using a French press homogenizer. The resulting product holds industrial value and may stimulate the immune system to combat respiratory pathogens prevalent in Kazakhstan's healthcare settings.
生物技术研究越来越侧重于开发新药，以应对医院中抗生素耐药菌株的增加。本研究旨在从医院各科室患者和医疗器械中分离出的抗生素耐药病原体中制备细菌裂解物。基于形态学、培养和生化特征以及使用BLAST算法的16S rRNA基因测序进行鉴定。使用迈尔斯和米斯拉方法评估菌株活力，同时使用琼脂扩散法评估对八类抗菌药物的敏感性以及不同培养物之间的生物安全性。从15个临床来源获得了25个纯分离株，并对其表型和基因型特性进行了研究。碳水化合物发酵试验证实这些分离株属于 、 、 、 、 、 、 和 属。这些培养物表现出良好的活力（10-10 CFU/mL）且彼此兼容。根据流行率和临床意义，选择了三种主要的医院病原体（12 BL、3 BL和24 BL）来开发细菌裂解物联合体。使用法国压榨均质器通过物理破碎制备裂解物。所得产品具有工业价值，可能会刺激免疫系统对抗哈萨克斯坦医疗环境中普遍存在的呼吸道病原体。
