Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Peshawar, KP, Pakistan.
Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan.
Curr Microbiol. 2024 Jan 31;81(3):83. doi: 10.1007/s00284-023-03610-8.
Zinc-solubilizing bacteria (ZSB) can convert insoluble zinc to an accessible form and increase Zn bioavailability in soil, which helps mitigate Zn deficiency in crops. In this study, different bacterial strains were screened for different Zn solubilization and plant growth promotion traits. Two bacterial strains, Acinetobacter pittii DJ55 and Stenotrophomonas maltophilia DJ24, were tested for their Zn-solubilizing potential on plate media, and both showed variable levels of Zn solubilization. The results showed that the bacterial strains applied to the plants in the pot experiment caused improvements in growth parameters compared to control conditions. DJ55, when applied with an insoluble source, enhanced plant height, leaf number, and leaf area compared to DJ24 and control conditions, while the maximum fruit weight was noticed in plants treated with ZnSO4. An increase in chlorophyll contents was noted in plants treated with ZnSO, while maximum carotenoid contents were observed in plants treated with DJ55 + ZnO when compared with their controls. Plants supplemented with ZnO and DJ55 showed higher zinc content and iron content as compared to their respective controls. The expression patterns of the SLZIP5 and SLZIP4 genes were changed in the root and shoot. Application of ZnO stimulates both gene expression and protein synthesis in tomato roots and shoots. Inoculation of tomato plants with ZSB and insoluble ZnO reduced the expression of the SLZIP5 and SLZIP4 genes in the root and shoot. In conclusion, both strains can be considered as potential zinc-solubilizing bioinoculants to promote the growth and production yield of tomato.
锌溶细菌（ZSB）可以将不溶性锌转化为可利用的形式，并增加土壤中锌的生物有效性，从而有助于缓解作物缺锌问题。在本研究中，筛选了不同的细菌菌株，以获得不同的溶锌和促进植物生长的特性。测试了两种细菌菌株，即不动杆菌 DJ55 和嗜麦芽寡养单胞菌 DJ24，以评估它们在平板培养基上的溶锌能力，结果表明这两种细菌菌株都具有不同程度的溶锌能力。盆栽实验结果表明，与对照条件相比，应用于植物的细菌菌株改善了生长参数。与 DJ24 和对照条件相比，当 DJ55 与不溶性锌源一起应用时，植物的株高、叶片数和叶面积都得到了提高，而在施用 ZnSO4 的植物中观察到最大的果实重量。施用 ZnSO4 的植物中，叶绿素含量增加，而在施用 DJ55+ZnO 的植物中，类胡萝卜素含量最高。与各自的对照相比，用 ZnO 和 DJ55 补充的植物显示出更高的锌含量和铁含量。SLZIP5 和 SLZIP4 基因的表达模式在根和茎中发生了变化。在番茄的根和茎中，施用 ZnO 刺激了这两个基因的表达和蛋白质合成。用 ZSB 和不溶性 ZnO 接种番茄植物会降低根和茎中 SLZIP5 和 SLZIP4 基因的表达。综上所述，这两种菌株都可以被认为是潜在的溶锌生物接种剂，以促进番茄的生长和产量。
