Gulcin İlhami, Mutlu Muzaffer, Bingol Zeynebe, Ozden Eda Mehtap, Mirzaee Ziba, Goren Ahmet C, Köksal Ekrem
Department of Chemistry, Faculty of Science, Ataturk University, Erzurum 25240, Türkiye.
Rectorate of Agri İbrahim Çeçen University, Agri 04100, Türkiye.
Molecules. 2025 Aug 14;30(16):3384. doi: 10.3390/molecules30163384.
This investigation explored the chemical constituents and biological activities of the steam-distilled oil of (SDOLU), employing sophisticated techniques including LC-HRMS, GC-MS, and GC-FID. The analysis identified a diverse array of 17 phenolic compounds, with linoleoyl chloride (64.05%) and linoleic acid (10.39%) as the major fatty acid components. The SDOLU demonstrated remarkable antioxidant capacity, effectively neutralizing free radicals in both DPPH (IC: 19.80 μg/mL) and ABTS (IC: 57.75 μg/mL) scavenging assays, alongside robust electron-donating activity in reducing ability tests. Moreover, the SDOLU showed significant inhibition of key enzymes implicated in metabolic and neurodegenerative disorders, including α-amylase (IC: 531.44 μg/mL), acetylcholinesterase (IC: 13.23 μg/mL), and carbonic anhydrase II (IC: 281.02 μg/mL). Collectively, these results highlight the SDOLU as a valuable natural source of multifunctional bioactivities with potential applications in combating oxidative stress and enzyme-related global diseases. Further studies are warranted to validate its therapeutic efficacy and expand its industrial utilization.
本研究采用液相色谱-高分辨质谱（LC-HRMS）、气相色谱-质谱联用（GC-MS）和气相色谱-氢火焰离子化检测器（GC-FID）等先进技术，对[植物名称]水蒸气蒸馏油（SDOLU）的化学成分和生物活性进行了探究。分析鉴定出了17种酚类化合物，其中亚油酰氯（64.05%）和亚油酸（10.39%）为主要脂肪酸成分。SDOLU表现出显著的抗氧化能力，在二苯基苦味酰基自由基（DPPH，半数抑制浓度IC：19.80 μg/mL）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS，半数抑制浓度IC：57.75 μg/mL）清除试验中能有效中和自由基，在还原能力测试中具有较强的供电子活性。此外，SDOLU对与代谢和神经退行性疾病相关的关键酶表现出显著抑制作用，包括α-淀粉酶（半数抑制浓度IC：531.44 μg/mL）、乙酰胆碱酯酶（半数抑制浓度IC：13.23 μg/mL）和碳酸酐酶II（半数抑制浓度IC：281.02 μg/mL）。总体而言，这些结果突出了SDOLU作为多功能生物活性物质的宝贵天然来源，在对抗氧化应激和酶相关全球性疾病方面具有潜在应用价值。有必要进一步开展研究以验证其治疗效果并扩大其工业应用。
