Orita Y, Imai N, Ueda N, Aoki K, Sugimoto K, Ando A, Fujiwara Y, Hirano S, Abe H
Nephron. 1977;19(1):19-25. doi: 10.1159/000180861.
Immunofluorescent staining of urinary casts of patients with chronic glomerulonephritis or with acute renal failure was performed. Urinary and cast Tamm-Horsfall mucoproteins were purified by a modified McQueen's method. Rabbits were immunized by both of these materials to obtain antisera. The antigenic specificity of the prepared material was examined by Ouchterlony gel diffusion method and immunoelectrophoresis. Indirect and direct immunofluorescent staining methods were done using Tamm-Horsfall mucoprotein antiserum and FITC-GARG, FITC-labelled IgG, IgA, IgM, C3 and albumin. Hyaline casts of both chronic glomerulonephritis and acute renal failure demonstrated fluorescence with Tamm-Horsfall mucoprotein antiserum. The nature of the granular casts of chronic glomerulonephritis seemed to be different from those of acute renal failure because the granules of the former fluoresced for some serum protein fractions, but the latter fluoresced only for Tamm-Horsfall mucoprotein. The absence of fluorescence with epithelial casts for any serum protein fractions implied a different pathogenesis for epithelial casts as compared to granular casts, contrary to Lippman's assumption. A routine technique of immunofluorescent staining of urinary casts was developed. The interpretation of urinary casts should be more specific by this new technique.
对慢性肾小球肾炎或急性肾衰竭患者的尿沉渣进行了免疫荧光染色。采用改良的麦奎因方法纯化尿沉渣及尿中Tamm-Horsfall粘蛋白。用这两种物质免疫兔子以获得抗血清。通过双向琼脂扩散法和免疫电泳检测所制备物质的抗原特异性。使用Tamm-Horsfall粘蛋白抗血清以及异硫氰酸荧光素标记的兔抗人γ球蛋白（FITC-GARG）、异硫氰酸荧光素标记的免疫球蛋白G（FITC-IgG）、免疫球蛋白A（FITC-IgA）、免疫球蛋白M（FITC-IgM）、补体C3和白蛋白进行间接和直接免疫荧光染色方法。慢性肾小球肾炎和急性肾衰竭的透明管型均显示与Tamm-Horsfall粘蛋白抗血清呈荧光反应。慢性肾小球肾炎颗粒管型的性质似乎与急性肾衰竭的不同，因为前者的颗粒对某些血清蛋白成分呈荧光反应，而后者仅对Tamm-Horsfall粘蛋白呈荧光反应。上皮管型对任何血清蛋白成分均无荧光反应，这意味着与颗粒管型相比，上皮管型的发病机制不同，这与李普曼的假设相反。开发了一种尿沉渣免疫荧光染色的常规技术。通过这项新技术，对尿沉渣的解读应该会更具特异性。
