Nityagovsky Nikolay N, Ananev Alexey A, Suprun Andrey R, Dneprovskaya Alina A, Kiselev Konstantin V, Aleynova Olga A
Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia.
Institute of the World Ocean, Far Eastern Federal University, 690090 Vladivostok, Russia.
Plants (Basel). 2025 Aug 14;14(16):2534. doi: 10.3390/plants14162534.
A wide range of microorganisms, including endophytes, frequently interact with forest trees. The role of endophytes in industrial conifers has not been fully investigated. The Yezo spruce is widely used for logging in Russia and Japan. In this work, the endophytic communities of bacteria and fungi in healthy needles, branches, and fresh wood of from Primorsky Territory were analyzed using metagenomic analysis. The results indicate that the diversity of endophytic communities in is predominantly influenced by the specific tree parts (for both bacteria and fungi) and by different tree specimens (for fungi). The most abundant bacterial classes were Alphaproteobacteria, Gammaproteobacteria and Actinobacteria. Functional analysis of KEGG orthologs (KOs) in endophytic bacterial community using PICRUSt2 and the PLaBAse PGPT ontology revealed that 59.5% of the 8653 KOs were associated with plant growth-promoting traits (PGPTs), mainly, colonization, stress protection, bio-fertilization, bio-remediation, vitamin production, and competition. Metagenomic analysis identified a high abundance of the genera and in , which are known for their potential growth-promoting activity in other coniferous species. The dominant fungal classes in were Dothideomycetes, Sordariomycetes, and Eurotiomycetes. Notably, the genus showed a pronounced increase in relative abundance within the fresh wood and needles of Yezo spruce, while displayed elevated abundance specifically in the fresh wood. It is known that some of these fungi exhibit antagonistic activity against phytopathogenic fungi. Thus, our study describes endophytic communities of the Yezo spruce and provides a basis for the production of biologicals with potential applications in forestry and agriculture.
包括内生菌在内的多种微生物经常与林木相互作用。内生菌在工业针叶树中的作用尚未得到充分研究。鱼鳞云杉在俄罗斯和日本广泛用于伐木。在这项工作中，利用宏基因组分析对来自滨海边疆区的健康针叶、树枝和新鲜木材中的细菌和真菌内生群落进行了分析。结果表明，鱼鳞云杉内生群落的多样性主要受特定树体部位（细菌和真菌均如此）以及不同树体样本（真菌）的影响。最丰富的细菌类群是α-变形菌纲、γ-变形菌纲和放线菌纲。使用PICRUSt2和PLaBAse PGPT本体对内生细菌群落中的KEGG直系同源物（KOs）进行功能分析发现，8653个KOs中有59.5%与促进植物生长的性状（PGPTs）相关，主要包括定殖、胁迫保护、生物施肥、生物修复、维生素生产和竞争。宏基因组分析确定了鱼鳞云杉中大量存在的属，这些属在其他针叶树种中以其潜在的促进生长活性而闻名。鱼鳞云杉中占主导地位的真菌类群是座囊菌纲、粪壳菌纲和散囊菌纲。值得注意的是，曲霉属在鱼鳞云杉的新鲜木材和针叶中的相对丰度显著增加，而青霉属则在新鲜木材中表现出较高的丰度。已知这些真菌中的一些对植物病原真菌具有拮抗活性。因此，我们研究描述了鱼鳞云杉的内生群落，并为生产在林业和农业中具有潜在应用价值的生物制品提供了依据。
