Godínez-Mendoza Pablo L, Rico-Chávez Amanda K, Carbajal-Valenzuela Ireri A, Contreras-Medina Luis M, Ocampo-Velázquez Rosalía V, Rico-García Enrique, Torres-Pacheco Irineo, Guevara-González Ramón G
Center of Applied Research in Biosystems (CARB-CIAB), School of Engineering, Autonomous University of Querétaro-Campus Amazcala, Carr. Amazcala-Chichimequillas Km 1.0, El Marqués 76265, Querétaro, Mexico.
Facultad de Química, Universidad Autónoma de Querétaro, Campus Centro Universitario, Cerro de las Campanas, s/n, Queretaro 76010, Querétaro, Mexico.
Plants (Basel). 2025 Aug 20;14(16):2591. doi: 10.3390/plants14162591.
Hydrogen peroxide (HO) application in several plant species has been widely studied as a plant biostimulant; however, the use of acoustic emissions related to hydric stress (MHAF) in biostimulating plants has not been widely studied, including the response of plants to the interaction of different stress factors. The aim of the present work was to evaluate the stress response in some morphological, biochemical, and molecular variables of the single or combined application of HO and MHAF in L. plants. Acoustic emission frequencies were obtained in a previous study where the frequencies came from plants submitted to medium hydric stress (MHAF). Our results showed that the combination of the two stressors evaluated has a possible synergistic effect on variables such as SOD activity and relative gene expressions of , , and MAPkinases (, , ), as well as an antagonistic effect for flavonoid content, DPPH, and ABTS free radical inhibition, and gene expression. MHAF showed increased plant height, PAL activity, and and gene upregulation, while HO increased POD activity and upregulated gene. These findings suggest possible stress response pathways that are activated and enhanced by the presence of these stress factors, both individually and in conjunction with one another, making it possible to use them as novel strategies for agricultural stress management.
过氧化氢（HO）作为一种植物生物刺激素，已在多种植物物种中得到广泛研究；然而，与水分胁迫相关的声发射（MHAF）在植物生物刺激方面的应用尚未得到广泛研究，包括植物对不同胁迫因素相互作用的响应。本研究的目的是评估HO和MHAF单独或联合应用对番茄植株某些形态、生化和分子变量的胁迫响应。声发射频率是在之前的一项研究中获得的，其中频率来自遭受中度水分胁迫的植株（MHAF）。我们的结果表明，所评估的两种胁迫源的组合对超氧化物歧化酶（SOD）活性以及PR1、PR2和促分裂原活化蛋白激酶（MPK1、MPK2、MPK3）的相对基因表达等变量可能具有协同效应，而对类黄酮含量、二苯基苦味酰基自由基（DPPH）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基抑制以及PR5基因表达具有拮抗效应。MHAF使株高增加、苯丙氨酸解氨酶（PAL）活性增强以及PR1和PR2基因上调，而HO使过氧化物酶（POD）活性增加并使PR5基因上调。这些发现表明，这些胁迫因素单独或相互结合时，可能会激活并增强一些胁迫响应途径，从而有可能将它们用作农业胁迫管理的新策略。
