Sarwar Humera, Martin Richard A, Coleman Heather M, Courtenay Aaron, Lowry Deborah
School of Pharmacy and Pharmaceutical Sciences, Ulster University, Cr Road, Coleraine BT52 1SA, UK.
College of Engineering and Physical Sciences, Aston University, Birmingham B4 7ET, UK.
Pathogens. 2025 Aug 1;14(8):760. doi: 10.3390/pathogens14080760.
This study explores the potential of biodegradable Bioglass 45S5 formulations as a dual-function approach for preventing and treating infections in orthopaedic surgery while addressing the growing concern of antimicrobial resistance (AMR). The research focuses on the development and characterisation of antibiotic-loaded BG45S5 formulations, assessing parameters such as drug loading efficiency, release kinetics, antimicrobial efficacy, and dissolution behaviour. Key findings indicate that the F2l-BG45S5-T-T-1.5 and F2l-BG45S5-T-V-1.5 formulations demonstrated controlled antibiotic release for up to seven days, with size distributions of D(10): 7.11 ± 0.806 µm, 4.96 ± 0.007 µm; D(50): 25.34 ± 1.730 µm, 25.20.7 ± 0.425 µm; and D(90): 53.7 ± 7.95 µm, 56.10 ± 0.579 µm, respectively. These formulations facilitated hydroxyapatite formation on their surfaces, indicative of osteogenic potential. The antimicrobial assessments revealed zones of inhibition against methicillin-susceptible (MSSA, ATCC-6538) measuring 20.3 ± 1.44 mm and 24.6 ± 1.32 mm, while for methicillin-resistant (MRSA, ATCC-43300), the inhibition zones were 21.6 ± 1.89 mm and 22 ± 0.28 mm, respectively. Time-kill assay results showed complete bacterial eradication within eight hours. Additionally, biocompatibility testing via MTT assay confirmed cell viability of >75%. In conclusion, these findings highlight the promise of antibiotic-loaded BG45S5 as a multifunctional biomaterial capable of both combating bone infections and supporting bone regeneration. These promising results suggest that in vivo studies should be undertaken to expedite these materials into clinical applications.
本研究探讨了可生物降解的45S5生物玻璃配方作为一种双功能方法在预防和治疗骨科手术感染方面的潜力，同时应对日益严重的抗菌药物耐药性（AMR）问题。该研究重点在于开发和表征负载抗生素的BG45S5配方，评估诸如载药效率、释放动力学、抗菌功效和溶解行为等参数。主要研究结果表明，F2l-BG45S5-T-T-1.5和F2l-BG45S5-T-V-1.5配方在长达七天的时间内实现了抗生素的控释，其尺寸分布分别为：D(10)： 7.11 ± 0.806 µm，4.96 ± 0.007 µm；D(50)：25.34 ± 1.730 µm，25.20.7 ± 0.425 µm；D(90)：53.7 ± 7.95 µm，56.10 ± 0.579 µm。这些配方促进了其表面羟基磷灰石的形成，表明具有成骨潜力。抗菌评估显示，对甲氧西林敏感菌（MSSA，ATCC-6538）的抑菌圈分别为20.3 ± 1.44 mm和24.6 ± 1.32 mm，而对耐甲氧西林金黄色葡萄球菌（MRSA，ATCC-43300）的抑菌圈分别为21.6 ± 1.89 mm和22 ± 0.28 mm。时间-杀菌试验结果表明，在八小时内可完全根除细菌。此外，通过MTT试验进行的生物相容性测试证实细胞活力>75%。总之，这些研究结果突出了负载抗生素的BG45S5作为一种多功能生物材料的前景，它既能对抗骨感染又能支持骨再生。这些有前景的结果表明，应开展体内研究，以便加快这些材料进入临床应用。
