Pescinelli Letícia M R, Longue Milena França, de Oliveira Giovana G F V, Thurler-Júnior Júlio C, Charret Thiago S, Nogueira Thalya S R, Pereira Mariana T M, Vieira Ivo J C, Abreu Lucas S, Pascoal Vinicius D B, Pascoal Aislan C R F
Research Laboratory of Natural Products and Bioactive Molecules, (Lab Nat-UFF) Nova Friburgo Health Institute, Fluminense Federal University-UFF, Nova Friburgo 28625-650, RJ, Brazil.
Multi-User Biomedical Research Laboratory, Nova Friburgo Health Institute, Fluminense Federal University-UFF, Nova Friburgo 28625-650, RJ, Brazil.
Pharmaceuticals (Basel). 2025 Aug 14;18(8):1199. doi: 10.3390/ph18081199.
This study analyzed the antiproliferative potential of L. leaf extracts against cervical cancer and non-cancerous cell lines. The extracts were prepared by maceration using hexane (EUH), dichloromethane (EUD), and ethyl acetate (EUA). Their cytotoxic potential was evaluated through MTT assays, wound healing assays, and flow cytometry. To identify classes of secondary metabolites, total phenolic and flavonoid contents were quantified using spectrophotometric methods, and individual metabolites were tentatively identified by LC-MS/MS. EUH, EUD. and EUA exhibited cytotoxicity in HeLa cells, with IC values of 63.03 μg/mL, 33.79 μg/mL, and 38.38 μg/mL, respectively. Due to their lower IC values, the EUD and EUA fractions were selected for further investigation. EUA and EUD inhibited cell migration at all the time points tested and altered the cell cycle. Twenty-eight compounds were tentatively identified in L. leaf extracts based on the interpretation of their fragmentation patterns and molecular formulas obtained from mass spectrometry. The EUD and EUA extracts appear to modulate the metabolism of cervical cancer cells, leading to cell cycle arrest and inhibition of cell migration. Flavonoids and other phenolic compounds are likely responsible for these observed biological effects.
本研究分析了L.叶提取物对子宫颈癌细胞系和非癌细胞系的抗增殖潜力。提取物通过用己烷（EUH）、二氯甲烷（EUD）和乙酸乙酯（EUA）浸渍制备。通过MTT试验、伤口愈合试验和流式细胞术评估它们的细胞毒性潜力。为了鉴定次生代谢物的类别，使用分光光度法对总酚和黄酮含量进行定量，并通过液相色谱-串联质谱法初步鉴定单个代谢物。EUH、EUD和EUA在HeLa细胞中表现出细胞毒性，IC值分别为63.03μg/mL、33.79μg/mL和38.38μg/mL。由于其较低的IC值，选择EUD和EUA馏分进行进一步研究。EUA和EUD在所有测试时间点均抑制细胞迁移并改变细胞周期。根据从质谱获得的碎片模式和分子式的解释，在L.叶提取物中初步鉴定出28种化合物。EUD和EUA提取物似乎调节子宫颈癌细胞的代谢，导致细胞周期停滞并抑制细胞迁移。黄酮类化合物和其他酚类化合物可能是这些观察到的生物学效应的原因。
