Graduate Program in Science and Biotechnology, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil.
Department of Basic Sciences, Fluminense Federal University (ISNF-UFF), Nova Friburgo, Rio de Janeiro, Brazil, (Instituto de Saúde de Nova Friburgo), Rua Dr Sìlvio Henrique Braune, 22, Centro, Nova Friburgo, Rio de Janeiro, Brazil.
Chem Biodivers. 2022 Aug;19(8):e202200114. doi: 10.1002/cbdv.202200114. Epub 2022 Jul 22.
Eugenia pyriformis, typically known as uvaia, ubaia, uvaieira, uvalha or uvalha-do-campo, is a plant representative of the Myrtaceae family. E. pyriformis decreased HeLa cells proliferation, can induce cell death and reduce cell migration that may be related to metastasis and induction of cell death by apoptosis in vitro assays. Its leaves are used in folk medicine for hypertension control, decreased cholesterol and uric acid, slimming, astringent, and digestive. In this work, the evaluation of the in vitro anticancer potential Cervical Cancer (HeLa cells) and phytochemical analysis in E. pyriformes was performed. It was possible to quantify phenolic compounds and total flavonoids and identify Chlorogenic acid, Quercetrin, and Myricitrin in this species. The crude extract and ethyl acetate fraction inhibited cell viability by 50 % in the dose of 44.42 μg/mL and 40.39 μg/mL, respectively. The induced effector caspase 3/7 activation, which results in apoptosis and the ethyl acetate fraction, decreases cell migration of cancer cell lines; it is responsible for the cleavage of several cellular proteins that will result in the classic phenotype of the apoptotic cell.
鸡蛋果，通常被称为 uvaia、ubaia、uvaieira、uvalha 或 uvalha-do-campo，是桃金娘科的一种植物代表。E. pyriformis 可降低 HeLa 细胞的增殖能力，能够诱导细胞死亡并减少细胞迁移，这可能与体外试验中的转移和细胞凋亡诱导有关。其叶子在民间医学中用于控制高血压、降低胆固醇和尿酸、减肥、收敛和消化。在这项工作中，评估了鸡蛋果对体外宫颈癌（HeLa 细胞）的抗癌潜力和植物化学分析。在该物种中，能够定量酚类化合物和总类黄酮，并鉴定出绿原酸、槲皮苷和杨梅素。粗提物和乙酸乙酯部分在 44.42μg/mL 和 40.39μg/mL 的剂量下分别抑制 50%的细胞活力。诱导效应半胱氨酸天冬氨酸酶 3/7 的激活导致细胞凋亡，而乙酸乙酯部分则降低癌细胞系的迁移能力；它负责切割几种细胞蛋白，从而导致凋亡细胞的典型表型。
