Azari-Dolatabad Nima, Benedetti Camilla, Velez Daniel Angel, Montoro Andrea Fernandez, Sadeghi Hafez, Residiwati Gretania, Leroy Jo L M R, Van Soom Ann, Pascottini Osvaldo Bogado
Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium; Department of Veterinary Sciences, Gamete Research Center, Veterinary Physiology and Biochemistry, University of Antwerp, Wilrijk, Belgium.
Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Anim Reprod Sci. 2023 Feb;249:107185. doi: 10.1016/j.anireprosci.2022.107185. Epub 2022 Dec 30.
The ovary and its hormones may have major effects on the in vitro developmental capacity of the oocytes it contains. We related intrinsic ovarian factors namely the presence of corpus luteum (CL) and/or dominant follicle (>8 mm) and the follicular count to cumulus expansion (CE), embryo development, and blastocyst quality in a bovine model. Cumulus-oocyte-complexes (COCs) were aspirated from follicles between 4 and 8 mm in diameter. In vitro embryo production was performed in a fully individual production system. The follicular fluid from which COCs were collected was pooled (per ovary) to evaluate the estrogen, progesterone, and insulin-like growth factor-1 (IGF-1) concentrations. Cumulus oocyte complexes collected from ovaries without a CL presented a greater CE than COCs derived from ovaries bearing CL. The absence of ovarian structures increased the blastocyst rate when compared to oocytes derived from ovaries with a CL, a dominant follicle, or both. Blastocysts derived from ovaries without a dominant follicle presented higher total cell numbers and a lower proportion of apoptosis than blastocysts derived from ovaries containing a dominant follicle. Cumulus oocyte complexes collected from ovaries with high follicular count resulted in higher cleavage than from ovaries with low follicular count, but the blastocyst rate was similar between groups. Ovaries bearing a CL had greater progesterone and IGF-1 follicular fluid concentrations in neighboring follicles than ovaries without a CL. Selection for bovine ovaries without CL or dominant follicle can have positive effects on CE, embryo development, and blastocyst quality in an individual embryo production system set-up.
卵巢及其激素可能对其所包含的卵母细胞的体外发育能力产生重大影响。在牛模型中，我们将卵巢内在因素（即黄体（CL）和/或优势卵泡（>8毫米）的存在以及卵泡计数）与卵丘扩展（CE）、胚胎发育和囊胚质量相关联。从直径4至8毫米的卵泡中抽吸卵丘-卵母细胞复合体（COC）。在完全个体化的生产系统中进行体外胚胎生产。将收集COC的卵泡液（每个卵巢）汇集起来，以评估雌激素、孕酮和胰岛素样生长因子-1（IGF-1）的浓度。从无CL的卵巢收集的卵丘-卵母细胞复合体比从有CL的卵巢获得的COC表现出更大的卵丘扩展。与从有CL、优势卵泡或两者皆有的卵巢获得的卵母细胞相比，无卵巢结构的情况下囊胚率增加。从无优势卵泡的卵巢获得的囊胚比从有优势卵泡的卵巢获得的囊胚具有更高的总细胞数和更低的凋亡比例。从卵泡计数高的卵巢收集的卵丘-卵母细胞复合体比卵泡计数低的卵巢产生更高的卵裂率，但两组之间的囊胚率相似。有CL的卵巢相邻卵泡中的孕酮和IGF-1卵泡液浓度比无CL的卵巢更高。在个体化胚胎生产系统设置中，选择无CL或优势卵泡的牛卵巢对卵丘扩展、胚胎发育和囊胚质量可能有积极影响。
