Wang Qian, Niu Xiaoyun, Huang Shuo, Di Dongliu, Su Beibei, Yuan Yangchen, Wu Yumeng, Huang Dazhuang
Hebei Key Laboratory of Floral Biological Breeding, Hebei Agricultural University, Baoding, China.
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, China.
Front Microbiol. 2025 Aug 12;16:1631852. doi: 10.3389/fmicb.2025.1631852. eCollection 2025.
Soil contamination with heavy metals (e.g., Pb, Cd) poses severe environmental risks due to industrialization. , a metal-tolerant woody plant, shows promise for phytoremediation, yet the synergistic role of nitrogen (N) fertilization in enhancing plant growth and soil remediation remains unclear. This study aims to elucidate how N fertilization optimizes remediation efficiency.
We applied integrated physiological and multi-omics approaches to assess N fertilization effects on growth, Pb/Cd uptake, and rhizosphere properties. Physiological metrics (biomass, metal accumulation) were combined with microbial community analysis (16S rRNA sequencing) and metabolomic profiling (LC-MS/GC-MS) of rhizosphere soils under varying N concentrations.
High N levels significantly increased plant biomass and Pb/Cd accumulation. Microbial diversity shifted, with enriched metal-mobilizing taxa. Metabolomics revealed elevated organic acids, correlating with improved metal bioavailability and soil health.
N fertilization synergistically enhances phytoremediation by: (1) stimulating plant growth and metal uptake, (2) reshaping rhizosphere microbiomes for metal mobilization, and (3) promoting chelating metabolite secretion. These findings provide actionable insights for optimizing N-assisted phytoremediation strategies.
由于工业化，土壤中重金属（如铅、镉）污染带来了严重的环境风险。作为一种耐金属的木本植物，在植物修复方面展现出前景，但氮肥在促进植物生长和土壤修复中的协同作用仍不明确。本研究旨在阐明氮肥如何优化修复效率。
我们应用综合生理学和多组学方法来评估氮肥对植物生长、铅/镉吸收以及根际特性的影响。将生理指标（生物量、金属积累）与不同氮浓度下根际土壤的微生物群落分析（16S rRNA测序）和代谢组学分析（液相色谱 - 质谱联用/气相色谱 - 质谱联用）相结合。
高氮水平显著增加了植物生物量和铅/镉积累。微生物多样性发生变化，金属活化类群增多。代谢组学显示有机酸含量升高，这与提高的金属生物有效性和土壤健康状况相关。
氮肥通过以下方式协同增强植物修复：（1）刺激植物生长和金属吸收；（2）重塑根际微生物群落以促进金属活化；（3）促进螯合代谢物分泌。这些发现为优化氮辅助植物修复策略提供了可操作的见解。
