Ren Hai-Yan, Liu Fang-Fang, Liang Chao-Peng, Rao Xiang-Jun
Anhui Province Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China.
Key Laboratory of Agri-products Quality and Biosafety (Anhui Agricultural University), Ministry of Education, Hefei, 230036, China.
Arch Microbiol. 2025 Aug 28;207(10):240. doi: 10.1007/s00203-025-04442-9.
Clonostachys rosea is a promising biocontrol fungus with multitrophic activity, yet effective native isolates with broad-spectrum potential remain limited. Here, we report the isolation and characterization of a novel soil-derived C. rosea strain, GY24, which exhibits potent entomopathogenic, antiphytopathogenic, and endophytic properties. Morphological and phylogenetic analyses confirmed its taxonomic identity, and optimal growth and sporulation were achieved on Oatmeal agar (OMA) medium. GY24 displayed high compatibility with chemical insecticides and strong tolerance to osmotic and cell wall stress, although it was sensitive to heat and UV exposure. In virulence assays against Galleria mellonella, GY24 caused up to 97% mortality within 84 h (LT₅₀ = 51.78 h), with characteristic mycosis symptoms and hyphal outgrowth. In dual-culture assays, GY24 significantly inhibited key phytopathogens, including Fusarium graminearum, Alternaria alternata, and Bipolaris maydis, and suppressed F. graminearum infection in cherry tomato fruit. Furthermore, GY24 established endophytic colonization in wheat, promoting plant growth and enhancing resistance to Fusarium-induced root rot. GY24-treated wheat displayed increased chlorophyll and lycopene levels and elevated superoxide dismutase (SOD) and catalase (CAT) activities, indicating improved photosynthetic performance and oxidative stress resilience. Collectively, these findings highlight the scientific significance of strain GY24 as a multitrophic biocontrol agent with integrated insecticidal, antifungal, and plant-beneficial functions. Its broad-spectrum activity and compatibility with agrochemicals make it a strong candidate for sustainable crop protection and integrated pest and disease management strategies.
粉红粘帚霉是一种具有多营养活性的有前景的生防真菌，但具有广谱潜力的有效本土分离株仍然有限。在此，我们报告了一种新的土壤来源的粉红粘帚霉菌株GY24的分离和特性，该菌株具有强大的昆虫病原性、抗植物病原性和内生特性。形态学和系统发育分析证实了其分类身份，并且在燕麦粉琼脂（OMA）培养基上实现了最佳生长和孢子形成。GY24与化学杀虫剂具有高度相容性，对渗透和细胞壁胁迫具有较强耐受性，尽管它对热和紫外线暴露敏感。在针对大蜡螟的毒力试验中，GY24在84小时内导致高达97%的死亡率（LT₅₀ = 51.78小时），伴有典型的真菌病症状和菌丝生长。在共培养试验中，GY24显著抑制了包括禾谷镰刀菌、链格孢和玉米小斑病菌在内的关键植物病原菌，并抑制了樱桃番茄果实中的禾谷镰刀菌感染。此外，GY24在小麦中建立了内生定殖，促进了植物生长并增强了对镰刀菌引起的根腐病的抗性。经GY24处理的小麦显示叶绿素和番茄红素水平增加以及超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性升高，表明光合性能和氧化应激恢复能力得到改善。总体而言，这些发现突出了菌株GY24作为具有综合杀虫、抗真菌和植物有益功能的多营养生防剂的科学意义。其广谱活性和与农用化学品的相容性使其成为可持续作物保护以及病虫害综合管理策略的有力候选者。
