一种基于体内吗啉代寡核苷酸的方法来阻断旁系特异性SUMO化修饰
A Vivo-Morpholino-Based Approach to Block Paralog-Specific SUMOylation.
作者信息
Orozco-Sepúlveda Rebeca, García-Morin Andrea, Gutiérrez-Zubiate Isabel, Rosas-Acosta Germán
机构信息
The University of Texas at El Paso (UTEP), El Paso, TX, USA.
出版信息
Methods Mol Biol. 2025;2957:191-206. doi: 10.1007/978-1-0716-4710-3_14.
We present an innovative method to specifically decrease SUMO2 SUMOylation by using a set of vivo-morpholinos inducing an alternative splicing event that produces a mRNA coding for SUMO2alpha, a non-conjugatable form of SUMO2. This method, also applicable to target SUMO1 and SUMO3, provides an alternative approach to assess the function of the various SUMO paralogs in different cells and the SUMO paralog preference of any given SUMO target.
我们提出了一种创新方法，通过使用一组体内吗啉代寡核苷酸来特异性降低SUMO2的SUMO化修饰，这些吗啉代寡核苷酸可诱导产生一种可变剪接事件，从而产生编码SUMO2α（一种不可共轭化的SUMO2形式）的mRNA。该方法也适用于靶向SUMO1和SUMO3，为评估不同细胞中各种SUMO旁系同源物的功能以及任何给定SUMO靶标的SUMO旁系同源物偏好提供了一种替代方法。
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