Ajabi Ardehjani Negar, Agha-Hosseini Marzieh, Shabani Nashtaei Maryam, Khodarahmian Mahshad, Seyyed Ebrahimi Shadi Sadat, Bagheri Mohammad Hossein, Fereidouni Farzane, Rastegar Tayebeh, Amidi Fardin
Department of Anatomical Sciences, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Department of Infertility, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Reprod Sci. 2025 Aug 28. doi: 10.1007/s43032-025-01967-8.
Polycystic ovary syndrome (PCOS) is a multifactorial endocrine disorder in women. Oxidative stress (OS) plays a pivotal role in the pathogenesis of PCOS. Resveratrol, as a natural polyphenol, has several beneficial therapeutic effects for women with PCOS. In this study, we investigated the antioxidant effect of resveratrol on OS markers in follicular fluid (FF) and granulosa cells (GCs) of patients with PCOS. A total of fifty-six patients with PCOS were randomly assigned to receive 800 mg/day of resveratrol or placebo for 60 days. The main focus was on evaluating OS markers, such as malondialdehyde (MDA) and total thiol (sulfhydryl) content in FF, along with assessing the levels of total antioxidant capacity (TAC) and total oxidant status (TOS) in GCs. Additionally, secondary outcomes included measuring the expression of genes associated with OS responses (Manganese superoxide dismutase (Mn-SOD), catalase (CAT), and uncoupling protein 2 (UCP2)), as well as the expression of UCP2 protein in granulosa cells. Compared with the placebo group, resveratrol reduced MDA and conversely significantly increased total thiol (P = 0.6694 and P = 0.0318, respectively) in FF. Resveratrol significantly reduced TOS and oxidative stress index (OSI) (P = 0.0299 and P = 0.0144, respectively) and significantly increased TAC (P = 0.0484) in GCs. There were significant increases in CAT and UCP2 expression after resveratrol consumption (P = 0.0158 and P = 0.0004, respectively). Although resveratrol increased Mn-SOD expression (P = 0.0914), its effects were not statistically significant. Resveratrol significantly affected UCP2 protein levels (P = 0.0030). These findings suggested that 60 days of supplementation with 800 mg/day resveratrol improves markers of OS in the FF and GCs of patients with PCOS. Trial registration number: http://www.irct.ir ; IRCT20221106056417N1; 2023 February 09.
多囊卵巢综合征（PCOS）是一种女性多因素内分泌疾病。氧化应激（OS）在PCOS的发病机制中起关键作用。白藜芦醇作为一种天然多酚，对PCOS女性具有多种有益的治疗作用。在本研究中，我们调查了白藜芦醇对PCOS患者卵泡液（FF）和颗粒细胞（GCs）中OS标志物的抗氧化作用。总共56例PCOS患者被随机分配接受每天800毫克白藜芦醇或安慰剂，持续60天。主要重点是评估OS标志物，如FF中的丙二醛（MDA）和总硫醇（巯基）含量，同时评估GCs中的总抗氧化能力（TAC）和总氧化状态（TOS）水平。此外，次要结果包括测量与OS反应相关的基因（锰超氧化物歧化酶（Mn-SOD）、过氧化氢酶（CAT）和解偶联蛋白2（UCP2））的表达，以及颗粒细胞中UCP2蛋白的表达。与安慰剂组相比，白藜芦醇降低了FF中的MDA，相反显著增加了总硫醇（分别为P = 0.6694和P = 0.0318）。白藜芦醇显著降低了GCs中的TOS和氧化应激指数（OSI）（分别为P = 0.0299和P = 0.0144），并显著增加了TAC（P = 0.0484）。服用白藜芦醇后，CAT和UCP2表达显著增加（分别为P = 0.0158和P = 0.0004）。虽然白藜芦醇增加了Mn-SOD表达（P = 0.0914），但其效果无统计学意义。白藜芦醇显著影响UCP2蛋白水平（P = 0.0030）。这些发现表明，每天补充800毫克白藜芦醇60天可改善PCOS患者FF和GCs中的OS标志物。试验注册号：http://www.irct.ir；IRCT20221106056417N1；2023年2月9日。
