一种人工蜱传脑炎病毒多表位免疫原的免疫原性可能取决于表位两侧的间隔区。

The Immunogenicity of an Artificial Tick-Borne Encephalitis Virus Polyepitope Immunogen May Depend on Spacers Flanking Epitope.

作者信息

Tigeeva E V, Shaburova E V, Antonets D V, Kisakov D N, Borgoyakova M B, Starostina E V, Yakovlev V A, Kisakova L A, Rudometova N B, Rudometov A P, Karpenko L I

机构信息

State Research Center of Virology and Biotechnology "VECTOR", Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia.

Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.

出版信息

Bull Exp Biol Med. 2025 Aug 29. doi: 10.1007/s10517-025-06462-3.

DOI:10.1007/s10517-025-06462-3

PMID:40877653

Abstract

The development of artificial polyepitope immunogens based on the conserved CD4 and CD8 epitopes from different virus proteins combined into a single molecule is a promising approach in the development of vaccines for activating a specific T-cell response. Selection of spacer sequences flanking the epitopes within the construct and providing the optimal processing of such immunogens within the cell is an important step in the design of such vaccines. We report the results of designing polyepitope T-cell immunogens of tick-borne encephalitis virus that differ in the presence or absence of optimally selected alanine spacers between epitopes, as well as the data of comparative analysis of immunogenic properties of DNA vaccines encoding the obtained polyepitope immunogens.

摘要

基于来自不同病毒蛋白的保守CD4和CD8表位组合成单个分子来开发人工多表位免疫原，是开发用于激活特异性T细胞应答的疫苗的一种有前景的方法。选择构建体内表位两侧的间隔序列并确保此类免疫原在细胞内得到最佳加工，是此类疫苗设计中的重要一步。我们报告了蜱传脑炎病毒多表位T细胞免疫原的设计结果，这些免疫原在表位之间存在或不存在最佳选择的丙氨酸间隔序列方面有所不同，同时还报告了编码所得多表位免疫原的DNA疫苗免疫原性特性的比较分析数据。