Schreiner J, Grabner G, Luger T A, Huber-Spitzy V, Haddad R, Stur M
Klin Monbl Augenheilkd. 1985 Nov;187(5):403-5. doi: 10.1055/s-2008-1054348.
A variety of distinct cells such as fibroblasts, epidermal cells, glioblastoma cells and corneal epithelial cells have been shown to produce Interleukin 1-like thymocyte-activating factors. Under in vitro conditions these cytokines influence a multiplicity of different cell types, including lymphocytes, fibroblasts, PMN and hepatocytes. In the present study human corneal fibroblasts, as well as the human epitheloid conjunctival Chang cell line were found to produce thymocyte-activating factors (TAF). The molecular weight of the corneal fibroblasts-TAF was found in the 670 kD region, with minor peaks of activity at 30, 17 and 4 kD. TAF from the Chang-cell line eluted at 15-25 kD. Chromatofocusing revealed the pI of the fibroblast-TAF to be at 6.7 and 5.2 and the pI of the conjunctival cells to be at 7.0, 5.3 and 5.0. The latter cells produced prostaglandin E2 upon stimulation. The immunomodulating properties of these cytokines make an important in vivo role in the pathogenesis of inflammatory corneal and conjunctival diseases probable.
多种不同的细胞，如成纤维细胞、表皮细胞、胶质母细胞瘤细胞和角膜上皮细胞，已被证明可产生白细胞介素1样胸腺细胞激活因子。在体外条件下，这些细胞因子会影响多种不同的细胞类型，包括淋巴细胞、成纤维细胞、多形核白细胞和肝细胞。在本研究中，发现人角膜成纤维细胞以及人上皮样结膜Chang细胞系可产生胸腺细胞激活因子（TAF）。角膜成纤维细胞-TAF的分子量在670 kD区域，在30、17和4 kD处有较小的活性峰。Chang细胞系的TAF在15-25 kD处洗脱。色谱聚焦显示成纤维细胞-TAF的pI为6.7和5.2，结膜细胞的pI为7.0、5.3和5.0。后者细胞在受到刺激时会产生前列腺素E2。这些细胞因子的免疫调节特性使其在体内可能在炎性角膜和结膜疾病的发病机制中发挥重要作用。
