Grabner G, Luger T A, Luger B M, Smolin G, Oh J O
Invest Ophthalmol Vis Sci. 1983 May;24(5):589-95.
Rabbit corneal epithelial cell cultures produce a cytokine (CETAF) that greatly enhances the proliferation of C3H/HeJ mouse thymocytes. The rabbit corneal cell line SIRC was used to generate CETAF activity in the culture supernatant. CETAF was then partially purified by Sephacryl S-200 gel filtration, where peaks of activity eluted in a molecular weight range of 95,000-55,000 (CETAF I) and 30,000-15,000 (CETAF II). Similar to the epidermal cell-derived thymocyte-activating factor (ETAF), CETAF (I and II) stimulated the growth of a human dermal fibroblast line (CRL 1445) in a dose-dependent manner, but failed to enhance the proliferation of an Interleukin 2 (IL 2)-dependent T-cell line (CT 6). Although CETAF did not exhibit any IL 2 activity, it clearly enhanced the IL 2 production by C3H/HeJ mouse splenocytes stimulated with suboptimal doses of lectins. Crude SIRC supernatants as well as the partially purified CETAF preparations showed a marked inhibition of polymorphonuclear neutrophil migration at high concentrations, but were significantly chemotactic when diluted samples were tested. CETAF release by SIRC cells was increased by stimulation with mitomycin C, phorbolmyristate acetate, hydroxyurea, silica, lipopolysaccaride B, and when the cells were cultured under serum-free conditions. These observations suggest that corneal epithelial cells may not only interact with the immune system in a way similar to keratinocytes, but may also stimulate corneal stromal cell through the production of CETAF.
兔角膜上皮细胞培养物产生一种细胞因子（CETAF），它能极大地增强C3H/HeJ小鼠胸腺细胞的增殖。兔角膜细胞系SIRC用于在培养上清液中产生CETAF活性。然后通过Sephacryl S - 200凝胶过滤对CETAF进行部分纯化，活性峰在分子量范围95,000 - 55,000（CETAF I）和30,000 - 15,000（CETAF II）处洗脱。与表皮细胞衍生的胸腺细胞激活因子（ETAF）类似，CETAF（I和II）以剂量依赖的方式刺激人皮肤成纤维细胞系（CRL 1445）的生长，但未能增强白细胞介素2（IL - 2）依赖性T细胞系（CT 6）的增殖。虽然CETAF没有表现出任何IL - 2活性，但它明显增强了用次优剂量凝集素刺激的C3H/HeJ小鼠脾细胞的IL - 2产生。粗制的SIRC上清液以及部分纯化的CETAF制剂在高浓度时对多形核中性粒细胞迁移有明显抑制作用，但在测试稀释样品时具有显著的趋化作用。丝裂霉素C、佛波酯肉豆蔻酸酯、羟基脲、二氧化硅、脂多糖B刺激以及细胞在无血清条件下培养时，SIRC细胞释放CETAF增加。这些观察结果表明，角膜上皮细胞不仅可能以类似于角质形成细胞的方式与免疫系统相互作用，还可能通过产生CETAF刺激角膜基质细胞。
