Peredo H, Borda E
Methods Find Exp Clin Pharmacol. 1985 Nov;7(11):573-7.
In vas deferens from acute and chronic diabetic rats a hypersensitivity to norepinephrine and acetylcholine was observed. The hypersensitivity to norepinephrine was markedly reduced by treatment with ouabain or verapamil. On the contrary, the response to acetylcholine was not modified by ouabain in the same concentration, but was inhibited by verapamil. Under normal conditions, ouabain and verapamil did not modify the stimulatory effect of either norepinephrine and acetylcholine; however increase of extracellular potassium concentrations potentiated the action of norepinephrine. The results suggest that ouabain-sensitive (Na+ + K+)ATPase exists in the rat vas deferens. In diabetic animals, the supersensitivity to norepinephrine is associated with an increase in membrane permeability to calcium ions that could be related to hypersensitivity of the enzyme. Under normal conditions it is possible to mimic the hypersensitivity to norepinephrine through an increase in the extracellular potassium concentration.
在急性和慢性糖尿病大鼠的输精管中，观察到对去甲肾上腺素和乙酰胆碱的超敏反应。用哇巴因或维拉帕米治疗后，对去甲肾上腺素的超敏反应明显降低。相反，相同浓度的哇巴因对乙酰胆碱的反应没有影响，但维拉帕米可抑制该反应。在正常情况下，哇巴因和维拉帕米不会改变去甲肾上腺素和乙酰胆碱的刺激作用；然而，细胞外钾浓度的增加会增强去甲肾上腺素的作用。结果表明，大鼠输精管中存在哇巴因敏感的（Na+ + K+）ATP酶。在糖尿病动物中，对去甲肾上腺素的超敏反应与膜对钙离子通透性的增加有关，这可能与该酶的超敏反应有关。在正常情况下，通过增加细胞外钾浓度可以模拟对去甲肾上腺素的超敏反应。
