使用在线固相萃取的高效液相色谱-串联质谱法分析血清5-羟色胺
Analysis of Serum Serotonin by High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using Online Solid-Phase Extraction.
作者信息
Bychenkov D V, Ioutsi V A, Kirilina I V, Ovcharov M V, Antsupova M A, Lubenskaya A K, Abdullaev L K, Pantsernaya S A, Mokrysheva N G
机构信息
National Medical Research Center for Endocrinology, Ministry of Health of the Russian Federation, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2025 Aug 29. doi: 10.1007/s10517-025-06474-z.
We propose a method for the quantitative determination of serotonin in blood serum using high-performance liquid chromatography with tandem mass spectrometry detection (HPLC-MS/MS) and online solid-phase extraction in a chromatographic system. This approach allows serotonin quantification in blood serum within a range of 0.5-2000 ng/ml, partially automates the sample preparation process, and minimizes the influence of matrix components. The method is designed for the quantitative measurement of serotonin levels in patients with various deviations of serotonin levels and associated diseases.
我们提出了一种使用高效液相色谱-串联质谱检测（HPLC-MS/MS）和色谱系统中的在线固相萃取技术定量测定血清中血清素的方法。该方法能够在0.5-2000 ng/ml范围内对血清中的血清素进行定量，部分自动化了样品制备过程，并将基质成分的影响降至最低。该方法旨在用于定量测量血清素水平存在各种偏差的患者以及相关疾病患者的血清素水平。
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