Gaugler Verena, Krusenbaum Lukas, Lange Esther, Pariyar Shyam, Fartyal Dhirendra, Becker Mathias, Wissuwa Matthias, Giehl Ricardo F H, Schaaf Gabriel, Kamleitner Marília
Department of Plant Nutrition, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, Germany.
Department of Physiology and Cell Biology, Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, Germany.
Methods Mol Biol. 2025;2972:221-233. doi: 10.1007/978-1-0716-4799-8_16.
Recent studies have established a key role of inositol pyrophosphates (PP-InsPs) in regulating phosphate (P) homeostasis across various organisms. In plants, PP-InsP levels are intricately linked to P status, with this reciprocal regulation being especially pronounced under fluctuating P conditions compared to stable nutrient sufficiency or deficiency. Here, we present a hydroponic cultivation protocol for Arabidopsis and rice, designed to simulate distinct and fluctuating P regimes. The composition of the nutrient solutions in these systems can be easily adjusted, enabling precise monitoring of changes in PP-InsP metabolism, under both steady-state and dynamic P conditions. Plants grown hydroponically under different P regimes can also be analyzed for phosphate starvation response (PSR) phenotypes and subjected to inositol phosphate (InsP)/PP-InsP extraction and quantification using methods detailed in other chapters of this special issue.
最近的研究已证实肌醇焦磷酸（PP-InsPs）在调节多种生物体的磷（P）稳态中起关键作用。在植物中，PP-InsP水平与磷状态密切相关，与稳定的养分充足或缺乏相比，这种相互调节在波动的磷条件下尤为明显。在这里，我们提出了一种用于拟南芥和水稻的水培栽培方案，旨在模拟不同的和波动的磷供应模式。这些系统中营养液的成分可以轻松调整，从而能够在稳态和动态磷条件下精确监测PP-InsP代谢的变化。在不同磷供应模式下进行水培生长的植物，还可以分析其磷饥饿响应（PSR）表型，并使用本期特刊其他章节中详述的方法进行肌醇磷酸（InsP）/PP-InsP的提取和定量分析。
