瘢痕疙瘩中LINE-1和Alu重复序列的甲基化

Methylation of LINE-1 and Alu repetitive sequence in keloid.

作者信息

Prabsattru Piyawan, Nakbua Tanchanok, Sengphairogh Suthida, Kitkumthorn Nakarin, Meevassana Jiraroch

机构信息

Program of Medical Science, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.

Center of Excellence in Burn and Wound Care, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.

出版信息

Sci Rep. 2025 Aug 30;15(1):31994. doi: 10.1038/s41598-025-17646-2.

DOI:10.1038/s41598-025-17646-2

PMID:40885823

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12398572/

Abstract

Keloid is a trauma-induced fibroproliferative condition characterized by excessive extracellular matrix (ECM) deposition and aberrant keloid fibroblast activation, leading to physical, psychological, functional, and cosmetic impairments. This study investigates DNA methylation alterations at Long Interspersed Nuclear Element-1 (LINE-1) and Alu repetitive elements in keloid tissues compared to normal skin tissues. Methylation levels and patterns were analyzed in keloid (n = 38) and normal skin tissues (n = 32). The results revealed significantly lower LINE-1 (P < 0.0001) and Alu (P = 0.0147) methylation levels in keloids, with hypomethylation inversely correlated with chronological age. Younger individuals exhibited higher methylation levels compared to older individuals. These findings offer critical insights into the epigenetic mechanisms underlying keloid formation and progression, providing a foundation for developing epigenetic-based therapeutic strategies.

摘要

瘢痕疙瘩是一种创伤诱导的纤维增生性疾病，其特征是细胞外基质（ECM）过度沉积和瘢痕疙瘩成纤维细胞异常激活，导致身体、心理、功能和美容方面的损害。本研究调查了瘢痕疙瘩组织与正常皮肤组织相比，长散在核元件1（LINE-1）和Alu重复元件的DNA甲基化改变。分析了瘢痕疙瘩（n = 38）和正常皮肤组织（n = 32）中的甲基化水平和模式。结果显示，瘢痕疙瘩中LINE-1（P < 0.0001）和Alu（P = 0.0147）的甲基化水平显著降低，低甲基化与实际年龄呈负相关。与年长者相比，年轻个体表现出更高的甲基化水平。这些发现为瘢痕疙瘩形成和进展的表观遗传机制提供了关键见解，为开发基于表观遗传学的治疗策略奠定了基础。