用 Alu siRNA 增加 Alu 元件的甲基化，防止 DNA 损伤。
Alu siRNA to increase Alu element methylation and prevent DNA damage.
机构信息
Inter-Department Program of Biomedical Sciences, Faculty of Graduate School, Chulalongkorn University, Bangkok, Thailand.
Program of Medical Science, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
出版信息
Epigenomics. 2018 Feb;10(2):175-185. doi: 10.2217/epi-2017-0096. Epub 2018 Jan 16.
UNLABELLED
Global DNA hypomethylation promoting genomic instability leads to cancer and deterioration of human health with age.
AIM
To invent a biotechnology that can reprogram this process.
METHODS
We used Alu siRNA to direct Alu interspersed repetitive sequences methylation in human cells. We evaluated the correlation between DNA damage and Alu methylation levels.
RESULTS
We observed an inverse correlation between Alu element methylation and endogenous DNA damage in white blood cells. Cells transfected with Alu siRNA exhibited high Alu methylation levels, increased proliferation, reduced endogenous DNA damage and improved resistance to DNA damaging agents.
CONCLUSION
Alu methylation stabilizes the genome by preventing accumulation of DNA damage. Alu siRNA could be useful for evaluating reprograming of the global hypomethylation phenotype in cancer and aging cells.
未加标签
全球 DNA 低甲基化促进基因组不稳定性，导致癌症，并随着年龄的增长而导致人类健康恶化。
目的
发明一种能够重新编程该过程的生物技术。
方法
我们使用 Alu siRNA 来指导人细胞中的 Alu 散布重复序列甲基化。我们评估了 DNA 损伤与 Alu 甲基化水平之间的相关性。
结果
我们观察到白细胞中 Alu 元件甲基化与内源性 DNA 损伤之间呈负相关。转染 Alu siRNA 的细胞表现出高 Alu 甲基化水平、增殖增加、内源性 DNA 损伤减少以及对 DNA 损伤剂的抵抗力增强。
结论
Alu 甲基化通过防止 DNA 损伤的积累来稳定基因组。Alu siRNA 可用于评估癌症和衰老细胞中全球低甲基化表型的重编程。
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