Salazar-García R, Palomares-Rius J E, Bajoub A, Ajal E A, Cantalapiedra-Navarrete C, Criado-Navarro I, Liébanas G, Castillo P, Archidona-Yuste A
https://ror.org/039vw4178Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Department of Crop Protection, Avenida Menéndez Pidal s/n, 14004 Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Spain.
Programa de Doctorado de Ingeniería Agraria, Alimentaria, Forestal y del Desarrollo Rural Sostenible. Universidad de Córdoba, Spain.
J Helminthol. 2025 Sep 1;99:e100. doi: 10.1017/S0022149X25100709.
The present study describes a new species, isolated from Meknès, Morocco, during nematode surveys conducted to investigate the biodiversity of plant-parasitic nematodes in Mediterranean olive groves and adjacent patches of natural vegetation. Application of integrative taxonomical approaches clearly verified that it is a new species designated herein as sp. nov., also representing the first report of the genus in Morocco. The new species is parthenogenetic, characterised by a short body 460 (373-528 μm); stylet delicate, relatively short, 8.7 (8.0-9.0) μm long with rounded basal knobs; six lines in the lateral fields; median bulb of pharynx oval, muscular and valvate; secretory-excretory pore located at the level of basal pharyngeal bulb region; vulva located at 79.9 (76.9-81.3) % of body length; relatively long post-vulval uterine sac 29.1 (20.0-39.0) μm; and a subcylindrical tail 24.4 (22.0-28.0) μm long, with a bluntly rounded tip. The results of molecular analysis of D2-D3 28S rRNA, ITS rRNA, partial 18S rRNA, and cytochrome oxidase c subunit 1 (COI) gene sequences support for the new species status and clearly separated sp. nov. from all other species within Phylogenetic analyses of ribosomal markers (D2-D3 28S rRNA and partial 18S rRNA) of this study confirms that is a monophyletic genus, clearly separated from other genera within Anguinoidea.
本研究描述了一个新物种，它是在对摩洛哥梅克内斯进行的线虫调查中分离得到的，该调查旨在研究地中海橄榄园及相邻自然植被斑块中植物寄生线虫的生物多样性。综合分类学方法的应用明确证实它是一个新物种，在此命名为sp. nov.，这也是该属在摩洛哥的首次报道。该新物种为孤雌生殖，其特征为身体短小，长460（373 - 528μm）；口针纤细，相对较短，长8.7（8.0 - 9.0）μm，基部瘤呈圆形；侧区有六条线；咽的中球呈椭圆形，肌肉质且有瓣膜；分泌排泄孔位于咽基部球区域水平；阴门位于体长的79.9（76.9 - 81.3）%处；阴门后子宫囊相对较长，为29.1（20.0 - 39.0）μm；尾部亚圆柱形，长24.4（22.0 - 28.0）μm，末端钝圆。对28S rRNA的D2 - D3、ITS rRNA、部分18S rRNA和细胞色素氧化酶c亚基1（COI）基因序列的分子分析结果支持该新物种的地位，并将sp. nov.与该属内的所有其他物种明显区分开来。本研究对核糖体标记（D2 - D3 28S rRNA和部分18S rRNA）的系统发育分析证实，该属是一个单系属，与滑刃总科内的其他属明显分开。
