Baraich Abdellah, Elbouzidi Amine, Jeddi Mohamed, Taibi Mohamed, Haddou Mounir, El Hachlafi Naoufal, Bellaouchi Reda, Mothana Ramzi A, Hawwal Mohammed F, Addi Mohamed, Rhazi Larbi, Benabbes Redouane, Asehraou Abdeslam, Jaouadi Bassem, Saalaoui Ennouamane
Laboratoire de Bioressources, Biotechnologie, Ethnopharmacologie et Santé (LBBES), Faculté des Sciences d'Oujda (FSO), Université Mohammed Premier (UMP), Bd Mohamed VI BP 717, Oujda, 60000, Morocco.
Laboratoire d'Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, 60000, Morocco.
Bioresour Bioprocess. 2025 Sep 1;12(1):94. doi: 10.1186/s40643-025-00892-7.
This study evaluated the antioxidant potential of a three-component mixture derived from Crocus sativus L. stigmas, leaves, and tepals, aiming to valorize these underutilized by-products. Hydroethanolic extracts were chemically profiled using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD), identifying major bioactive compounds including crocin (11.04%), rutin (22.32%), and ellagic acid (62.47%). Antioxidant capacity was assessed using DPPH and ABTS assays, yielding IC values ranging from 88.67 ± 0.83 µg/mL to 291.41 ± 0.91 µg/mL for DPPH, and from 116.76 ± 1.31 µg/mL to 286.87 ± 0.25 µg/mL for ABTS. A simplex-centroid mixture design was employed to determine optimal component ratios. The best-performing DPPH mixture (43% stigmas, 35% leaves, 22% tepals) achieved an IC of 97.36 ± 1.23 µg/mL, closely matching the predicted 95.96 µg/mL. Similarly, the optimal ABTS blend (45% stigmas, 35% leaves, 20% tepals) reached an IC of 110.59 ± 6.82 µg/mL, aligning with the predicted 106.31 µg/mL. Both combinations outperformed standard antioxidants like BHT and ascorbic acid. These findings support the synergistic use of saffron by-products in food preservation, nutraceuticals, and biopharmaceuticals, offering an eco-friendly, cost-effective solution aligned with circular economy principles.
本研究评估了源自藏红花柱头、叶子和花被片的三组分混合物的抗氧化潜力，旨在使这些未充分利用的副产品具有价值。采用带二极管阵列检测的高效液相色谱法（HPLC-DAD）对水乙醇提取物进行化学分析，鉴定出主要生物活性化合物，包括藏红花素（11.04%）、芦丁（22.32%）和鞣花酸（62.47%）。使用DPPH和ABTS测定法评估抗氧化能力，DPPH的IC值范围为88.67±0.83μg/mL至291.41±0.91μg/mL，ABTS的IC值范围为116.76±1.31μg/mL至286.87±0.25μg/mL。采用单纯形重心混合物设计来确定最佳组分比例。表现最佳的DPPH混合物（43%柱头、35%叶子、22%花被片）的IC值为97.36±1.23μg/mL，与预测的95.96μg/mL非常接近。同样，最佳的ABTS混合物（45%柱头、35%叶子、20%花被片）的IC值为110.59±6.82μg/mL，与预测的106.31μg/mL相符。这两种组合均优于BHT和抗坏血酸等标准抗氧化剂。这些发现支持在食品保鲜、营养保健品和生物制药中协同使用藏红花副产品，提供了一种符合循环经济原则的环保、经济高效的解决方案。
