Asgharian Heshmat, Kammarchedu Vinay, Gu Scott J, Ebrahimi Aida
Res Sq. 2025 Aug 20:rs.3.rs-7274589. doi: 10.21203/rs.3.rs-7274589/v1.
Real-time, multiplexed monitoring of wound infection biomarkers is essential for early detection of infection and inflammation, as well as for evaluating wound healing progression. However, existing biosensing technologies lack the sensitivity, specificity, and integration needed to meet these clinical demands. To address current limitations in wound monitoring, we developed a portable and multimodal sensor system capable of simultaneously detecting uric acid (UA), phenazine-1-carboxylic acid (PCA), interleukin-6 (IL-6), and pH. The device integrates laser-induced graphene (LIG) electrodes with differential pulse voltammetry for UA and PCA sensing, open-circuit potential sensing using polyaniline-modified LIG for pH, and an extended-gate field-effect transistor-based component for IL-6 detection. We validated the performance of the sensors in phosphate-buffered saline and a wound simulating medium, demonstrating accurate, multiplexed detection across clinically relevant ranges. Furthermore, experiments with an agar-based wound model highlighted the system's promise for future application in wound monitoring. A custom-designed wireless analyzer was integrated to enable real-time, multiplexed data acquisition at the point-of-care. This minimally invasive platform offers a comprehensive solution for continuous wound assessment, with potential applications in infection detection, inflammatory disease monitoring, and next-generation smart wound dressings.
实时、多重监测伤口感染生物标志物对于早期检测感染和炎症以及评估伤口愈合进程至关重要。然而，现有的生物传感技术缺乏满足这些临床需求所需的灵敏度、特异性和集成度。为了解决当前伤口监测中的局限性，我们开发了一种便携式多模态传感器系统，能够同时检测尿酸（UA）、吩嗪-1-羧酸（PCA）、白细胞介素-6（IL-6）和pH值。该设备将激光诱导石墨烯（LIG）电极与用于UA和PCA传感的差分脉冲伏安法、使用聚苯胺修饰的LIG进行pH值的开路电位传感以及用于IL-6检测的基于扩展栅场效应晶体管的组件集成在一起。我们在磷酸盐缓冲盐水和伤口模拟介质中验证了传感器的性能，证明了在临床相关范围内能够进行准确的多重检测。此外，基于琼脂的伤口模型实验突出了该系统在伤口监测未来应用中的前景。集成了定制设计的无线分析仪，以实现即时护理点的实时、多重数据采集。这个微创平台为连续伤口评估提供了一个全面的解决方案，在感染检测、炎症性疾病监测和下一代智能伤口敷料方面具有潜在应用。
