Li Xinjie, Hu Linping, Xu Feng, Yu Wenbang, Wu Yixuan, Deng Junhongyu, Wei Zihan, Shi Guoyue, Zhang Min
School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, Dongchuan Road 500, Shanghai, 200241, China.
Department of Pharmacy, 6th People's Hospital South Campus, Shanghai Jiao Tong University, Shanghai, 201499, China.
Talanta. 2025 May 12;295:128312. doi: 10.1016/j.talanta.2025.128312.
Vancomycin (Van), a widely utilized glycopeptide antibiotic in clinical settings, necessitates precise bloodstream concentration monitoring due to its narrow therapeutic window, ensuring drug efficacy while preventing adverse effects. In this work, we have engineered an extended-gate field-effect transistor (EG-FET) sensor tailored for vancomycin detection. This novel sensor configuration comprises a detachable multi-doped graphene EG electrode sensing element paired with a commercial field-effect transistor (FET). The EG electrode design integrates a sophisticated multistage doping process, incorporating MnO and Au nanoparticles into laser-induced graphene (LIG), thereby augmenting both functional and electrical characteristics of LIG. To achieve specific recognition, a vancomycin aptamer is immobilized onto the electrode surface, enabling selective binding with vancomycin and translating this interaction into a measurable electrical signal. This collaborative mechanism empowers the EG-FET sensor to exhibit exceptional sensitivity and selectivity towards vancomycin. Notably, the sensor demonstrates a wide linear response ranging from 1 nM to 100 μM, spanning the entire therapeutic window of vancomycin (6-35 μM), boasting an impressive detection limit of 0.187 nM. We have innovated a portable wireless sensing system, coupled with a Janus membrane for expedited plasma separation, consolidating a portable platform dedicated to vancomycin sensing. Furthermore, we have realized the detection of vancomycin concentration in patient's blood using this sensor, and the results are reliable. This comprehensive study underscores the immense potential of multi-doped graphene EG-FET sensors in the realm of antibiotic detection, thereby contributing a pivotal tool towards the realization of precision medicine strategies.
万古霉素（Van）是临床环境中广泛使用的糖肽类抗生素，由于其治疗窗狭窄，需要精确监测血药浓度，以确保药物疗效并防止不良反应。在这项工作中，我们设计了一种用于检测万古霉素的扩展栅场效应晶体管（EG-FET）传感器。这种新型传感器配置包括一个可拆卸的多掺杂石墨烯EG电极传感元件与一个商用场效应晶体管（FET）配对。EG电极设计集成了复杂的多级掺杂工艺，将MnO和Au纳米颗粒掺入激光诱导石墨烯（LIG）中，从而增强了LIG的功能和电学特性。为了实现特异性识别，将万古霉素适配体固定在电极表面，使其能够与万古霉素选择性结合，并将这种相互作用转化为可测量的电信号。这种协同机制使EG-FET传感器对万古霉素表现出卓越的灵敏度和选择性。值得注意的是，该传感器在1 nM至100 μM的范围内呈现出宽线性响应，涵盖了万古霉素的整个治疗窗（6-35 μM），检测限低至0.187 nM。我们创新了一种便携式无线传感系统，并结合了用于快速血浆分离的Janus膜，巩固了一个专门用于万古霉素传感的便携式平台。此外，我们已经使用该传感器实现了对患者血液中万古霉素浓度的检测，结果可靠。这项全面的研究强调了多掺杂石墨烯EG-FET传感器在抗生素检测领域的巨大潜力，从而为实现精准医疗策略贡献了一个关键工具。
