Tayeh Malatee, Sama-Ae Imran, Wisessombat Sueptrakool, Sianglum Wipawadee
Department of Medical Technology and Hematology and Transfusion Science Research Center, Walailak University, Nakhon Si Thammarat, Thailand.
Department of Medical Technology and Center of Excellence Research for Melioidosis and Microorganisms, Walailak University, Nakhon Si Thammarat, Thailand.
Scientifica (Cairo). 2025 Aug 21;2025:5676577. doi: 10.1155/sci5/5676577. eCollection 2025.
Endophytic fungi are microorganisms that infect living plant tissues internally without producing obvious symptoms of infection, existing in a symbiotic relationship with plants for a portion of their life cycle. Currently, endophytic fungi serve as alternate sources for the production of new bioactive chemicals with great efficacy. This study aimed to examine the antimigration and anti-invasion capabilities of the endophytic fungus extract, isolated from L., utilizing and methods. This study isolated the endophytic fungus from the leaves of L. LC-MS analysis revealed fifty-five active components within the extract. Ten compounds exhibited favorable results in the assessment. Computational predictions indicate that tajixanthone methanoate (-8.80 kcal/mol) and aspernigerin (-12.95 kcal/mol) exhibited high binding affinity against MMP-2. The extract demonstrated antiproliferative activity with an IC value of 286.36 ± 122.57 μg/mL. The extract, at noncytotoxic concentrations, reduced the migration and invasion of A549 cells in a dose-dependent manner. Furthermore, extract demonstrated a marked reduction in MMP-2 activity. According to these results, the compounds may serve as antimigration and anti-invasion agents by inhibiting the MMP-2 protein. The results demonstrated that extract derived from L. inhibited A549 cell migration and invasion via reducing MMP-2 activity. The findings indicated that extract derived from L. may be utilized for the treatment of lung cancer.
内生真菌是一种微生物，它在植物活组织内部感染，却不产生明显的感染症状，在其生命周期的一部分时间内与植物形成共生关系。目前，内生真菌是生产具有高效能的新型生物活性化学品的替代来源。本研究旨在利用Transwell和划痕实验方法，检测从L.中分离出的内生真菌提取物的抗迁移和抗侵袭能力。本研究从L.的叶片中分离出内生真菌。液相色谱-质谱联用（LC-MS）分析显示提取物中有55种活性成分。在Transwell实验评估中，10种化合物呈现出良好的结果。计算预测表明，甲酸太济黄烷酮（-8.80 kcal/mol）和曲霉尼汀（-12.95 kcal/mol）对基质金属蛋白酶-2（MMP-2）表现出高结合亲和力。该提取物表现出抗增殖活性，IC值为286.36±122.57 μg/mL。在非细胞毒性浓度下，该提取物以剂量依赖的方式减少了A549细胞的迁移和侵袭。此外，该提取物使MMP-2活性显著降低。根据这些结果，这些化合物可能通过抑制MMP-2蛋白而作为抗迁移和抗侵袭剂。结果表明，从L.中提取的提取物通过降低MMP-2活性抑制了A549细胞的迁移和侵袭。研究结果表明，从L.中提取的提取物可用于治疗肺癌。
