Bak Hyeok-Jin, Kim Eun-Ji, Lee Ji-Hyeon, Chang Sungyul, Kwon Dongwon, Im Woo-Jin, Hwang Woon-Ha, Chang Jae-Ki, Chung Nam-Jin, Sang Wan-Gyu
National Institute of Crop and Food Science, Rural Development Administration, Wanju-gun, Republic of Korea.
National Institute of Horticultural and Herbal Science, Rural Development Administration, Muan-gun, Republic of Korea.
Front Plant Sci. 2025 Aug 14;16:1611653. doi: 10.3389/fpls.2025.1611653. eCollection 2025.
INTRODUCTION: Rising global populations and climate change necessitate increased agricultural productivity. Most studies on rice panicle detection using imaging technologies rely on single-time-point analyses, failing to capture the dynamic changes in panicle coverage and their effects on yield. Therefore, this study presents a novel temporal framework for rice phenotyping and yield prediction by integrating high-resolution RGB imagery with deep learning-based semantic segmentation. METHODS: High-resolution RGB images of rice canopies were acquired over two growing seasons. We evaluated five semantic segmentation models (DeepLabv3+, U-Net, PSPNet, FPN, LinkNet) to effectively delineate rice panicles. Time-series panicle coverage data, extracted from the segmented images, were fitted to a piecewise function to model their growth and decline dynamics. This process distilled key predictive parameters: (maximum panicle coverage), (growth rate), (time of maximum growth rate), a (decline rate), and (transition point). These parameters served as predictors in four machine learning regression models (PLSR, RFR, GBR, and XGBR) to estimate yield and its components. RESULTS: In panicle segmentation, DeepLabv3+ and LinkNet achieved superior performance (mIoU > 0.81). Among the piecewise function parameters, K showed the strongest positive correlation with Yield and Grain Number (GN) ( = 0.87 and = 0.85, respectively), while was strongly negatively correlated with the Filled Grain Ratio (FGR) ( = -0.71). For yield prediction, the RFR and XGBR models demonstrated the highest performance (R= 0.89). SHAP analysis quantified the relative importance of each parameter for predicting yield components. DISCUSSION: This framework proves to be a powerful tool for quantifying rice developmental dynamics and accurately predicting yield using readily available RGB imagery. It holds significant potential for advancing both precision agriculture and crop breeding efforts.
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