Ha Annie, Arbet Jaron, Qiu Zhuyu, Khoo Amanda, Main Brian P, Govindarajan Meinusha, Waas Matthew, Liu Stanley K, Semmes O John, Nyalwidhe Julius O, Boutros Paul C, Kislinger Thomas
Department of Medical Biophysics, University of Toronto, Toronto, Canada.
Princess Margaret Cancer Centre, University Health Network, Toronto, Canada.
bioRxiv. 2025 Aug 19:2025.08.14.670396. doi: 10.1101/2025.08.14.670396.
Urine is an attractive biomarker analyte for non-invasive longitudinal monitoring of health and disease, particularly for diseases of the genitourinary tract, like prostate and bladder cancer. The composition of an individual's urine reflects both genetic and lifestyle characteristics that differ across geographies and populations, like diet, hydration and other socio-economic factors. While men of African ancestry have elevated prostate cancer risk, it is unclear to what extent this influences urinary biomarkers. We therefore quantified the urinary proteomes of 329 localized prostate cancer patients: 135 self-identifying as White and 194 self-identifying as Black. We identified 110 proteins that significantly differed between these groups after controlling for age, PSA, and cISUP. Immune pathways were particularly dysregulated. The urinary proteome of Black patients harboured more features of aggressive cancers than those of grade- and PSA-matched White patients. These observations highlight the importance of controlling for race- and ancestry-associated differences in the development of urinary biomarkers.
尿液是用于健康和疾病无创纵向监测的一种有吸引力的生物标志物分析物，特别是对于泌尿生殖道疾病，如前列腺癌和膀胱癌。个体尿液的成分反映了不同地理区域和人群之间的遗传和生活方式特征，如饮食、水合作用和其他社会经济因素。虽然非洲裔男性患前列腺癌的风险较高，但尚不清楚这在多大程度上影响尿液生物标志物。因此，我们对329例局限性前列腺癌患者的尿液蛋白质组进行了定量分析：135例自我认定为白人，194例自我认定为黑人。在控制年龄、前列腺特异性抗原（PSA）和国际泌尿病理学会（ISUP）分级后，我们鉴定出110种在这些组之间有显著差异的蛋白质。免疫途径尤其失调。与年龄和PSA匹配的白人患者相比，黑人患者的尿液蛋白质组具有更多侵袭性癌症的特征。这些观察结果突出了在尿液生物标志物开发中控制种族和血统相关差异的重要性。
