Baniya Anil, Zayed Omar, Achi Perla, Perfetto Pierluigi, Dillman Adler
Department of Nematology, University of California, Riverside, 900 University Ave., Riverside, CA 92521.
Department of Botany and Plant Science, University of California, Riverside, 900 University Ave., Riverside, CA 92521.
J Nematol. 2025 Aug 31;57(1):20250031. doi: 10.2478/jofnem-2025-0031. eCollection 2025 Feb.
, or tree houseleek (), is a bushy, perennial succulent and a popular ornamental plant in regions such as California, New Zealand, Australia, Sicily, Gibraltar, and Chile. It features rosettes of soft, waxy leaves at the tips of sparsely branched or occasionally single, bare stems. It is drought-tolerant and has a variety of colors and forms, making it a popular ornamental plant. In July 2024, a diseased plant was submitted by a home gardener from Los Angeles County, California, to the Department of Nematology at the University of California, Riverside (UCR), for diagnosis. Root galls were observed on the plant, and further examination revealed high numbers of root-knot nematodes ( sp.). Molecular species identification was conducted using ribosomal DNA, mitochondrial haplotyping, and species-specific primer techniques, including the TRNAH/MHR106 and MORF/MTHIS primer sets, along with -specific primers (MIF/MIR). Amplification and sequencing of the marker genes identified the root-knot nematode infecting as . To our knowledge, this study presents the first report of infecting worldwide.
或树长生草(),是一种丛生的多年生多肉植物,在加利福尼亚、新西兰、澳大利亚、西西里岛、直布罗陀和智利等地区是一种受欢迎的观赏植物。它在稀疏分枝或偶尔单生的光秃茎的顶端有莲座状柔软、蜡质的叶子。它耐旱,有多种颜色和形态,使其成为一种受欢迎的观赏植物。2024年7月,加利福尼亚州洛杉矶县的一位家庭园艺师将一株患病的植物提交给加利福尼亚大学河滨分校(UCR)的线虫学系进行诊断。在该植物上观察到根瘤,进一步检查发现大量根结线虫(种)。使用核糖体DNA、线粒体单倍型分析和物种特异性引物技术进行分子物种鉴定,包括TRNAH/MHR106和MORF/MTHIS引物组,以及特异性引物(MIF/MIR)。标记基因的扩增和测序确定感染的根结线虫为。据我们所知,本研究是全球范围内首次报道感染。