Lee Joonwoo, Choi Jinmi, Park Jeongeun, Lee Seonduk, Cho Eun-Jung, Gwon Youngdae
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea.
Sungkyunkwan University School of Pharmacy, Suwon, 16419, Republic of Korea.
Genome Biol. 2025 Sep 2;26(1):264. doi: 10.1186/s13059-025-03757-6.
Cellular senescence is accompanied by extensive genomic reorganization, such as senescence-associated heterochromatin foci and expanded interchromatin compartments, to ultimately affect gene expression. Here, we demonstrate that chromatin structural changes in senescent cells drive significant alterations in the phase behavior and motility of paraspeckles, a type of interchromatin compartment condensate. We observe increased numbers, size, and elongation of paraspeckles harboring NONO and NEAT1_2, driven by elevated levels of those components, consistent with the micellization model of longitudinal growth rather than condensate coalescence. Enhanced paraspeckle motility is associated with HP1α-mediated heterochromatin condensation and interchromatin expansion found in cellular senescence.
细胞衰老伴随着广泛的基因组重组，如衰老相关异染色质聚集体和扩展的染色质间区室，最终影响基因表达。在此，我们证明衰老细胞中的染色质结构变化驱动了副斑点（一种染色质间区室凝聚物）的相行为和运动性的显著改变。我们观察到，含有NONO和NEAT1_2的副斑点数量增加、尺寸增大且伸长，这是由这些组分水平升高所驱动的，这与纵向生长的胶束化模型一致，而非凝聚物聚结。副斑点运动性增强与细胞衰老中HP1α介导的异染色质凝聚和染色质间扩展有关。
