Jaglarz-Biały Katarzyna, Konturek Aleksander, Jagła Joanna, Orzeł Agnieszka, Król-Dyrek Katarzyna, Frączek Justyna, Krośniak Mirosław
Jagiellonian University Medical College, Doctoral School of Medical and Health Sciences, Kraków, Poland; Department of Clinical Oncology, St Lukas Hospital, Tarnów, Poland;
Department of Endocrine Surgery, Third Chair of General Surgery, Jagiellonian University Medical College, Kraków, Poland.
Folia Med Cracov. 2024 Dec 30;64(4):47-57. doi: 10.24425/fmc.2024.153272.
Anthocyanins are a group of organic compounds believed to show anti-inflammatory, anti-proliferative, antioxidant, pro-apoptotic effects. For these reasons, anthocyanins may potentially be lied in the cancer chemoprevention as well as in providing a metabolic support during oncological treatment.
The aim of this study is to compare the antioxidant activity and analyze the content of bioactive substances in selected freeze-dried fruit species in order to determine the optimal source of anthocyanins for subsequent clinical trials involving patients undergoing treatment for colon cancer.
The study employed freeze-dried fruits of chokeberry, blackberry, strawberry and raspberries: black, yellow and red. The total antioxidant activity was determined using the FRAP, the DPPH method and total polyphenol by UV-VIS spectrophotometer.
Chokeberry exhibited the highest antioxidant activity, as measured by the FRAP method, followed by black raspberry, blackberry, strawberry, yellow and red raspberry. The highest content of polyphenols was found in chokeberry and black raspberry, while the other fruits had relatively lower levels. Black raspberry fruits contained the highest concentration of anthocyanins. The highest concentration of vitamin C was detected in yellow raspberry fruits.
Based on biochemical analysis and considering organoleptic properties, particularly taste and the potential for good tolerance of the formulation by patients, freeze-dried fruits of black raspberry and strawberry were selected for clinical study on the use of anthocyanins in the nutritional and metabolic support of patients undergoing treatment for colorectal cancer.
花青素是一类有机化合物,被认为具有抗炎、抗增殖、抗氧化、促凋亡作用。基于这些原因,花青素可能潜在地用于癌症化学预防以及在肿瘤治疗期间提供代谢支持。
本研究的目的是比较选定的冻干水果品种的抗氧化活性并分析其生物活性物质的含量,以确定用于后续涉及结肠癌治疗患者的临床试验的最佳花青素来源。
本研究采用了黑果腺肋花楸、黑莓、草莓以及红、黄两种树莓的冻干果实。使用铁离子还原抗氧化能力(FRAP)法、1,1-二苯基-2-三硝基苯肼(DPPH)法测定总抗氧化活性,并通过紫外可见分光光度计测定总多酚含量。
通过FRAP法测定,黑果腺肋花楸表现出最高的抗氧化活性,其次是黑树莓、黑莓、草莓、黄树莓和红树莓。多酚含量最高的是黑果腺肋花楸和黑树莓,而其他水果的含量相对较低。黑树莓果实中花青素浓度最高。黄树莓果实中检测到的维生素C浓度最高。
基于生化分析并考虑感官特性,特别是味道以及患者对制剂良好耐受性的可能性,选择黑树莓和草莓的冻干果实用于关于花青素在结直肠癌治疗患者的营养和代谢支持中的应用的临床研究。