Pangesti Kartissa, Anggani Haru Setyo, Bachtiar Endang Winiati
Department of Orthodontics, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
J Adv Pharm Technol Res. 2025 Jul-Sep;16(3):151-155. doi: 10.4103/JAPTR.JAPTR_256_24. Epub 2025 Aug 9.
Stainless steel temporary anchorage device (SS TAD) has toxic risk due to the content that may be released when exposed to the oral environment, and the mouthwash being used. This research aims to analyze the cytotoxicity of SS TAD and measure the inflammation level in cells after exposure to three types of mouthwash. SS TADs ( = 28) were divided into four groups ( = 7 per group) and immersed in the following mouthwash solutions for 90 days. The resulting eluates were then applied to BHK-21 fibroblast cell cultures and incubated for 24 h. Matrix metalloproteinase-8 (MMP-8) levels in the supernatants on days 1 and 7 were measured using enzyme-linked immunosorbent assay MMP-8 kit. BHK-21 fibroblast cells showed significant differences in reactivity ( < 0.05) after exposure to SS TAD eluate in povidone-iodine (PVP-I) and chitosan mouthwash, compared to control groups without SS TAD. Viability test revealed significant differences ( < 0.05) after exposure to SS TAD eluate in PVP-I mouthwash compared to PVP-I alone. The reactivity of BHK-21 fibroblast cells exposed to fluoride and distilled water was not significantly different ( > 0.05) from control groups without SS TAD. The viability of BHK-21 fibroblast cells exposed to fluoride and distilled water did not differ significantly from control groups without SS TAD. MMP-8 levels differed significantly between SS TAD eluate groups ( < 0.05) on day 1 and day 7, with day 7 levels significantly higher than day 1. The most recommended mouthwash is chitosan for TAD SS users rather than fluoride and PVP-I.
不锈钢临时锚固装置（SS TAD）由于在口腔环境中暴露时可能释放的成分以及正在使用的漱口水而存在毒性风险。本研究旨在分析SS TAD的细胞毒性，并测量暴露于三种漱口水后细胞中的炎症水平。将28个SS TAD分为四组（每组7个），并浸入以下漱口水溶液中90天。然后将所得洗脱液应用于BHK - 21成纤维细胞培养物中并孵育24小时。使用酶联免疫吸附测定MMP - 8试剂盒测量第1天和第7天上清液中基质金属蛋白酶-8（MMP - 8）的水平。与没有SS TAD的对照组相比，BHK - 21成纤维细胞在暴露于聚维酮碘（PVP - I）和壳聚糖漱口水的SS TAD洗脱液后反应性有显著差异（P<0.05）。活力测试显示，与单独使用PVP - I相比，暴露于PVP - I漱口水的SS TAD洗脱液后有显著差异（P<0.05）。暴露于氟化物和蒸馏水的BHK - 21成纤维细胞的反应性与没有SS TAD的对照组没有显著差异（P>0.05）。暴露于氟化物和蒸馏水的BHK - 21成纤维细胞的活力与没有SS TAD的对照组没有显著差异。在第1天和第7天，SS TAD洗脱液组之间的MMP - 8水平有显著差异（P<0.05），第7天的水平显著高于第1天。对于使用SS TAD的用户，最推荐的漱口水是壳聚糖，而不是氟化物和PVP - I。
