Jiang Xing, Wang Xiaoli, Shen Song, Hou Shangguo, Yu Chen
School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou 511442, China.
Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, China.
Research (Wash D C). 2025 Sep 1;8:0865. doi: 10.34133/research.0865. eCollection 2025.
Dynamic chromatin 3-dimensional (3D) conformation is a key mechanism regulating gene expression and cellular function during development and disease. Elucidating the structure, functional dynamics, and spatiotemporal organization of the 3D genome requires integrating multiple experimental approaches, including chromatin conformation capture techniques, precise genome manipulation tools, and advanced imaging technologies. Notably, CRISPR/Cas systems have emerged as a revolutionary genome-editing platform, offering unprecedented opportunities for manipulating 3D genome organization and investigating disease mechanisms. This review systematically examines recent advances in CRISPR-based mammalian 3D genome engineering and explores the therapeutic potential of 3D genome engineering strategies in disease intervention.
动态染色质三维（3D）构象是发育和疾病过程中调节基因表达和细胞功能的关键机制。阐明3D基因组的结构、功能动力学和时空组织需要整合多种实验方法，包括染色质构象捕获技术、精确的基因组操作工具和先进的成像技术。值得注意的是，CRISPR/Cas系统已成为一个革命性的基因组编辑平台，为操纵3D基因组组织和研究疾病机制提供了前所未有的机会。本文综述系统地研究了基于CRISPR的哺乳动物3D基因组工程的最新进展，并探讨了3D基因组工程策略在疾病干预中的治疗潜力。
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