Arcos-Reyes Yesenia, Rivera Gildardo, Marchat Laurence A, Salas-Benito Juan, Mandujano-Lázaro Gilberto, Monzón-Gualito Moisés, Ramírez-Moreno Esther
Instituto Politécnico Nacional, Escuela Nacional de Medicina y Homeopatía, Laboratorio de Biomedicina Molecular 2, México City, México.
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Laboratorio de Biotecnología Farmacéutica, Reynosa, México.
PLoS One. 2025 Sep 3;20(9):e0330860. doi: 10.1371/journal.pone.0330860. eCollection 2025.
Obesity and overweight are two highly prevalent conditions worldwide, which can lead to death or produce chronic and degenerative diseases. The search for alternative therapies to control these morbidities can involve the study of metabolites obtained from plants. Particularly, pentacyclic triterpenes produce an antiadipogenic effect by affecting the expression of master regulators of adipogenesis and their signaling pathways, including LKB1-AMPK pathway. In this work, we evaluated the effect of the pentacyclic triterpene isoarborinol on adipogenesis in 3T3-L1 cells. This molecule inhibits differentiation and decreases lipid accumulation during cell differentiation in a dose-dependent manner, deregulates the expression of C/EBPβ, C/EBPδ, C/EBPα, PPARγ and SREBP-1C, causes an increase in the phosphorylation of LKB1 and AMPK, as well as a down regulation of the lipogenic factors ACC1, FAS and FABP4. These findings show that the antiadipogenic effect of isoarborinol is associated with the activation of LKB1-AMPK, leading to changes in the expression of master regulators of adipogenesis and lipogenic factors.
肥胖和超重是全球范围内两种高度普遍的状况，可导致死亡或引发慢性退行性疾病。寻找控制这些病症的替代疗法可能涉及对从植物中获取的代谢物的研究。特别是，五环三萜通过影响脂肪生成的主要调节因子及其信号通路，包括LKB1-AMPK通路，产生抗脂肪生成作用。在这项工作中，我们评估了五环三萜异乔木萜醇对3T3-L1细胞脂肪生成的影响。该分子以剂量依赖性方式抑制细胞分化过程中的分化并减少脂质积累，使C/EBPβ、C/EBPδ、C/EBPα、PPARγ和SREBP-1C的表达失调，导致LKB1和AMPK的磷酸化增加，以及脂肪生成因子ACC1、FAS和FABP4的下调。这些发现表明，异乔木萜醇的抗脂肪生成作用与LKB1-AMPK的激活有关，导致脂肪生成主要调节因子和脂肪生成因子的表达发生变化。
