Sharmanov T Sh, Siniavskiĭ Iu A, Aĭdarkhanov B B, Nadirov N K, Lenskaia E G
Vopr Pitan. 1985 Nov-Dec(6):47-50.
August male rats were kept for 90 days on one of the following diets: balanced semisynthetic diet with casein as a source of protein (group 1), amino acid balanced diet with casein replaced by gluten (group 2), a diet with excess of polyunsaturated fatty acids (group 3), with vitamin E deficiency (group 4), and polyunbalanced diet, comprising a combination of amino acid imbalance, excess of polyunsaturated acids, and vitamin E deficiency (group 5). Structural and functional parameters of Ca2+ transport (Ca2+ accumulation rate, activity of Ca2+-ATPase, Ca/ATP ratio), content of lipid fractions and accumulation of malonic dialdehyde were studied in sarcoplasmatic reticulum (SR) fragments from rat hind limb muscles in the course of ascorbate-dependent lipid peroxidation. Reduction of Ca2+ absorption rate, Ca2+-ATPase, and Ca/ATP ratio in SR membranes were observed in groups 2-5. In parallel, decreased phospholipid and triglyceride levels and increased content of free fatty acids and cholesterol in SR membranes were established.
将八月龄雄性大鼠分别用以下其中一种日粮喂养90天：以酪蛋白作为蛋白质来源的平衡半合成日粮（第1组）、用谷蛋白替代酪蛋白的氨基酸平衡日粮（第2组）、多不饱和脂肪酸过量的日粮（第3组）、维生素E缺乏的日粮（第4组）以及包含氨基酸失衡、多不饱和脂肪酸过量和维生素E缺乏组合的多不平衡日粮（第5组）。在抗坏血酸依赖性脂质过氧化过程中，研究了大鼠后肢肌肉肌浆网（SR）片段中Ca2+转运的结构和功能参数（Ca2+积累速率、Ca2+-ATP酶活性、Ca/ATP比值）、脂质组分含量以及丙二醛的积累情况。在第2 - 5组中观察到SR膜中Ca2+吸收率、Ca2+-ATP酶和Ca/ATP比值降低。同时，还发现SR膜中磷脂和甘油三酯水平降低，游离脂肪酸和胆固醇含量增加。
