Nadirov N K, Lenskaia E G, Tokmurzin Zh U, Aĭdarkhanov B B, Siniavskiĭ Iu A
Vopr Pitan. 1986 Jan-Feb(1):47-51.
For 90 days male August rats were kept on 5 diets: (I) balanced semisynthetic, (II) with amino acid unbalance, (III) with excess polyunsaturated fatty acids (PUFA), (IV) with vitamin E deficiency, and (V) polyunbalanced (amino acid unbalance, excess PUFA, vitamin E deficiency). In liver microsomes, the authors studied the kinetics of malonic dialdehyde accumulation in the course of NADPH-dependent lipid peroxidation (LP) and microviscosity of the lipid phase of microsomal membranes according to eximerization of the pyrene fluorescent hydrophobic probe. The microsomes of the animals fed diets I, III and IV showed on the average a 50 to 55% increase in the rate of MDA formation, whereas those of rats on diet V a 78% increase as compared with appropriate characteristics in the animals fed diet I. A good correlation was established between the decrease in the pyrene eximerization rate and accumulation of lipid peroxides: r = 0.09 (P less than 0.05). The possibility of affecting LP in the membranes by the goal oriented modification of the diet is discussed. The participation of proteins, lipids and tocopherol in the maintenance of the membrane structure is described.
将雄性八月龄大鼠分别喂以5种日粮90天：（I）平衡半合成日粮，（II）氨基酸不平衡日粮，（III）多不饱和脂肪酸（PUFA）过量日粮，（IV）维生素E缺乏日粮，以及（V）多因素不平衡日粮（氨基酸不平衡、PUFA过量、维生素E缺乏）。作者研究了肝微粒体中，在NADPH依赖的脂质过氧化（LP）过程中丙二醛积累的动力学，以及根据芘荧光疏水探针的准分子形成情况研究微粒体膜脂质相的微粘度。喂食日粮I、III和IV的动物的微粒体，丙二醛形成速率平均增加50%至55%，而喂食日粮V的大鼠的微粒体，与喂食日粮I的动物的相应特征相比，丙二醛形成速率增加78%。芘准分子形成速率的降低与脂质过氧化物的积累之间建立了良好的相关性：r = 0.09（P < 0.05）。讨论了通过有针对性地改变日粮来影响膜中LP的可能性。描述了蛋白质、脂质和生育酚在维持膜结构中的作用。
