Gebrekidan Atsbha Gebreslasie, Zhang Yong, Chen Julian
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Tigray Agricultural Research Institute, Mekelle 7000, Ethiopia.
Biology (Basel). 2025 Aug 1;14(8):964. doi: 10.3390/biology14080964.
Disparities in the functional classification of secretory genes among aphid taxa may be attributed to variations in coding sequences and gene expression profiles. However, the driving factors that regulate sequence evolution remain unclear. This study aimed to investigate the differences in coding sequences and expression patterns of secretory genes between the rose grain aphid () and the pea aphid (), with a particular focus on their roles in evolutionary adaptations and functional diversity. The study involved the rearing of aphids, RNA extraction, de novo transcriptome assembly, functional annotation, secretory protein prediction, and comparative analysis of coding sequences and expression patterns across various functional categories using bioinformatics tools. The results revealed that metabolic genes exhibited greater coding sequence divergence, indicating the influence of positive selection. Moreover, significant expression divergence was noted among functional categories, particularly in metabolic and genetic information processing genes, which exhibited higher variability. This study enhances our understanding of the molecular mechanisms that contribute to phenotypic and genetic diversity among aphid species. This study elucidates the relationship between variations in coding sequences and differences in gene expression among functional categories, thereby establishing a foundation for future studies on gene evolution in response to environmental pressures.
蚜虫类群中分泌基因功能分类的差异可能归因于编码序列和基因表达谱的变化。然而，调控序列进化的驱动因素仍不清楚。本研究旨在调查蔷薇长管蚜（）和豌豆蚜（）之间分泌基因编码序列和表达模式的差异，特别关注它们在进化适应和功能多样性中的作用。该研究涉及蚜虫饲养、RNA提取、从头转录组组装、功能注释、分泌蛋白预测，以及使用生物信息学工具对不同功能类别的编码序列和表达模式进行比较分析。结果表明，代谢基因表现出更大的编码序列差异，表明受到正选择的影响。此外，在功能类别之间观察到显著的表达差异，特别是在代谢和遗传信息处理基因中，这些基因表现出更高的变异性。本研究增进了我们对导致蚜虫物种表型和遗传多样性的分子机制的理解。本研究阐明了编码序列变异与功能类别间基因表达差异之间的关系，从而为未来研究基因如何响应环境压力而进化奠定了基础。
