Joo Soobin, Cho Jaemin, Kim Taewon
Department of Ocean Sciences, Inha University, Incheon 22212, Republic of Korea.
Program in Biomedical Science and Engineering, Inha University, Incheon 22212, Republic of Korea.
Biology (Basel). 2025 Jul 31;14(8):962. doi: 10.3390/biology14080962.
Anthropogenic vibrational disturbances in the marine environment can affect benthic organisms, but these effects on marine animals remain poorly understood. To examine whether anthropogenic substrate-borne vibrations induce physiological stress in the white-clawed fiddler crab (), individuals were exposed to vibrations at 120 Hz and 250 Hz (~100 dB re 1 µm/s), and physiological indicators were measured. Lactate and ATP concentrations in the leg muscle were measured, and heat shock protein 70 kDa (HSP70) gene expression in the hepatopancreas was analyzed using RT-PCR with newly designed primers. At 120 Hz, ATP and lactate levels in the leg muscle did not differ significantly between the exposure and control groups. However, at 250 Hz, ATP levels were lower and lactate levels were higher in the exposure group compared to the control. HSP70 gene expression in the hepatopancreas did not differ significantly between the exposure and control groups at either frequency, although one individual exposed to 250 Hz exhibited markedly elevated expression, inducing higher expression variability in the exposed group. These results suggest that anthropogenic vibrational pollution may induce physiological stress in , and that such physiological indices could serve as biomarkers for assessing vibroacoustic pollution on marine animals.
海洋环境中的人为振动干扰会影响底栖生物，但这些对海洋动物的影响仍知之甚少。为了研究人为产生的基质传播振动是否会在白爪招潮蟹中引发生理应激，将个体暴露于120赫兹和250赫兹（约100分贝，相对于1微米/秒）的振动中，并测量生理指标。测量腿部肌肉中的乳酸和三磷酸腺苷（ATP）浓度，并使用新设计的引物通过逆转录聚合酶链反应（RT-PCR）分析肝胰腺中70千道尔顿热休克蛋白（HSP70）的基因表达。在120赫兹时，暴露组和对照组腿部肌肉中的ATP和乳酸水平没有显著差异。然而，在250赫兹时，与对照组相比，暴露组的ATP水平较低，乳酸水平较高。在两种频率下，暴露组和对照组肝胰腺中的HSP70基因表达均无显著差异，尽管一只暴露于250赫兹的个体表现出明显升高的表达，导致暴露组的表达变异性更高。这些结果表明，人为振动污染可能会在白爪招潮蟹中引发生理应激，并且这些生理指标可以作为评估海洋动物振动声学污染的生物标志物。
