Reda Fayiz M, Kamal Mahmoud, Mahmoud Hemat K, Rudayni Hassan A, Allam Ahmed A, Khafaga Asmaa F, Khan Mohammad M H, Mohamed Reda S
Poultry Department, Faculty of Agriculture, Zagazig University, Zagazig, 44519, Egypt.
Animal Production Research Institute, Agricultural Research Center, Dokki, Giza 12618, Egypt.
Poult Sci. 2025 Aug 23;104(11):105718. doi: 10.1016/j.psj.2025.105718.
This study aimed to assess the effect of supplementing Arbor Acre broiler chick diets with a blend of peppermint and clove oils on growth efficiency, blood indicators, and intestinal microbial communities. In a randomized experimental design, 250 unsexed, one-day-old Arbor Acre broiler chicks were allocated into 5 groups. Each group included 5 replicates, each containing 10 birds. The control group administered a basal diet devoid of oils, whereas the remaining groups were provided with meals enriched with 150, 300, 450, or 600 mg/kg of the oil blend. The findings indicated significant variations (P ≤ 0.05) among the treatment groups during weeks 3 to 6-supplementation with the oil blend led to increased body weight (BW) and body weight gain (BWG). Compared to the control, birds receiving the oil consumed less feed intake (FI) and exhibited an improved feed conversion ratio (FCR). Additionally, the oil-supplemented diets significantly (P ≤ 0.05) decreased abdominal fat and altered the relative weights of the gizzard, giblets, spleen, thymus, and bursa. Except for hemoglobin, all hematological indicators were substantially influenced by the oil supplementation. Biochemical analysis revealed significant reductions (P ≤ 0.05) in serum levels of alanine transaminase (ALT), aspartate transaminase (AST), urea, creatinine, cholesterol, triglycerides, low-density lipoprotein (LDL), and glucose. Conversely, total protein, albumin, globulin, and high-density lipoprotein (HDL) levels significantly increased (P ≤ 0.05). Oil supplementation also enhanced immune response and antioxidant enzyme activities, as evidenced by significant increases (P ≤ 0.05) in total oxidant capacity (TAC), superoxide dismutase (SOD), reduced glutathione (GSH), and catalase (CAT), along with a notable decrease in malondialdehyde (MDA) levels. Moreover, birds fed diets containing the oil blend exhibited changes in digestive enzyme activity and blood mineral levels. Microbiological assessments showed decreased counts of Salmonella and E. coli in the oil-treated groups. In conclusion, dietary supplementation with peppermint and clove oils improved growth performance, enhanced gut microbiota balance, and positively influenced carcass traits and biochemical markers in broiler chickens.
本研究旨在评估在爱拔益加肉鸡日粮中添加薄荷油和丁香油混合物对生长效率、血液指标和肠道微生物群落的影响。在随机实验设计中，将250只一日龄未区分性别的爱拔益加肉鸡分为5组。每组包括5个重复，每个重复包含10只鸡。对照组给予不含油的基础日粮，而其余组则提供添加了150、300、450或600 mg/kg油混合物的日粮。研究结果表明，在第3至6周的油混合物添加期内，各处理组之间存在显著差异（P≤0.05），添加油混合物可增加体重（BW）和体重增加量（BWG）。与对照组相比，摄入油的鸡采食量（FI）减少，饲料转化率（FCR）提高。此外，添加油的日粮显著（P≤0.05）降低了腹部脂肪，并改变了肌胃、内脏、脾脏、胸腺和法氏囊的相对重量。除血红蛋白外，所有血液学指标均受油添加的显著影响。生化分析显示，血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、尿素、肌酐、胆固醇、甘油三酯、低密度脂蛋白（LDL）和葡萄糖水平显著降低（P≤0.05）。相反，总蛋白、白蛋白、球蛋白和高密度脂蛋白（HDL）水平显著升高（P≤0.05）。添加油还增强了免疫反应和抗氧化酶活性，总抗氧化能力（TAC）、超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）和过氧化氢酶（CAT）显著增加（P≤0.05），丙二醛（MDA）水平显著降低，证明了这一点。此外，饲喂含油混合物日粮的鸡消化酶活性和血液矿物质水平发生了变化。微生物学评估显示，油处理组中沙门氏菌和大肠杆菌的数量减少。总之，在肉鸡日粮中添加薄荷油和丁香油可改善生长性能，增强肠道微生物群平衡，并对胴体性状和生化指标产生积极影响。
